異檸檬酸脫氫酶1(IDH1)重組蛋白

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產品名稱: 異檸檬酸脫氫酶1(IDH1)重組蛋白

產品型號: IDH1

產品展商: HZbscience

產品文檔: 無相關文檔

簡單介紹

異檸檬酸脫氫酶1(IDH1)重組蛋白敏性高，高效性，原裝進口，吸附均勻、吸附性好，回收利用率高、可靠性強，無效包退包換，各種種屬重組蛋白產品齊全，質量可靠。異檸檬酸脫氫酶1(IDH1)重組蛋白熱穩定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產生。保質期內，在適當的條件下存儲，損失率低于5%。

異檸檬酸脫氫酶1(IDH1)重組蛋白 的詳細介紹

異檸檬酸脫氫酶1(IDH1)重組蛋白
適用生物   Mus musculus (Mouse，小鼠)
異檸檬酸脫氫酶1(IDH1)重組蛋白
產品應用   SDS-PAGE; WB; ELISA; IP.   規格   50ug
分子量   57.6kDa   價格   請咨詢當地代理商
純度   > 95%
來源   原核表達

Recombinant Isocitrate Dehydrogenase 1, Soluble (IDH1)

FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!

Organism species	Mus musculus (Mouse)
Product No.	HZH839Mu01
Source	Prokaryotic expression
Host	E.coli
Purity	> 95%
UOM	50ug
Predicted Molecular Mass	57.6kDa
Predicted isoelectric point	6.5異檸檬酸脫氫酶1(IDH1)重組蛋白
Applications	SDS-PAGE; WB; ELISA; IP.
Endotoxin Level	<1.0EU per 1μg (determined by the LAL method)

Subcellular Location	Cytoplasm
Residues	Ile99~Thr325 (Accession # O88844) with two N-terminal Tags, His-tag and GST-tag
Formulation	Supplied as lyophilized form in 20mM Tris, 500mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.

SEQUENCES

The target protein is fused with two N-terminal Tags, His-tag and GST-tag, its sequence is listed below.
MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTLEV LFQGPLGSEF-IR NILGGTVFRE AIICKNIPRL VTGWVKPIII GRHAYGDQYR ATDFVVPGPG KVEITYTPKD GTQKVTYMVH DFEEGGGVAM GMYNQDKSIE DFAHSSFQMA LSKGWPLYLS TKNTILKKYD GRFKDIFQEI YDKKYKSQFE AQKICYEHRL IDDMVAQAMK SEGGFIWACK NYDGDVQSDS VAQGYGSLGM MTSVLICPDG KTVEAEAAHG TVTRHYRMYQ KGQET

USAGE

Reconstitute in sterile PBS, pH7.2-pH7.4.

STORAGE AND STABILITY

異檸檬酸脫氫酶1(IDH1)重組蛋白Storage: Avoid repeated freeze/thaw cycles. Store at 2-8^oC for one month. Aliquot and store at -80^oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37^oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

About the MARKER (complimentary)

Effective Size Range: 10kDa to 70kDa.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
異檸檬酸脫氫酶1(IDH1)重組蛋白Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
Ready-to-use: No need to heat, dilute or add reducing agents before use.

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