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產(chǎn)品名稱: 腫瘤蛋白p53(TP53)多克隆抗體
產(chǎn)品型號: TP53
產(chǎn)品展商: HZbscience
產(chǎn)品文檔: 無相關(guān)文檔
簡單介紹
腫瘤蛋白p53(TP53)多克隆抗體是針對TP53的兔多克隆抗體。在**組織化學(xué)染色和**印跡實(shí)驗(yàn)中能識別TP53。腫瘤蛋白p53(TP53)多克隆抗體豐富產(chǎn)品組合，其中包括超過 42,000 多種高品質(zhì)抗體，并配套提供種類齊全的抗體相關(guān)產(chǎn)品。 我們的抗體測定結(jié)果經(jīng)全球上千次的引用驗(yàn)證，有助于您在一系列抗體應(yīng)用中獲得優(yōu)異的實(shí)驗(yàn)結(jié)果。
腫瘤蛋白p53(TP53)多克隆抗體
的詳細(xì)介紹
腫瘤蛋白p53(TP53)多克隆抗體
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來源多抗制備
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宿主兔
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效價(jià)-
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Ig類型IgG
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純化方式抗原特異性親和純化
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標(biāo)記物無標(biāo)記物
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**原腫瘤蛋白p53(TP53)重組蛋白
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緩沖液成份磷酸鹽緩沖液（pH7.4,含有0.02% NaN3和50%甘油）
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性狀液體
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濃度200ug/ml
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且適物種-
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應(yīng)用腫瘤蛋白p53(TP53)重組蛋白WB; ICC; IHC-P; IHC-F; ELISA; IP; IF; FCM.
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Polyclonal Antibody to Tumor Protein p53 (TP53)
Organism Species: Homo sapiens (Human)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
11th Edition (Revised in May, 2016)
[ PROPERTIES ]
Source: Polyclonal antibody preparation
Host: Rabbit
Purification: Antigen-specific Affinity Chromatography. Traits: Liquid
Concentration: 200μg/mL
UOM: 100μg
Applications: WB; ICC; IHC-P; IHC-F; ELISA; IP; IF; FCM. [ IMMUNOGEN ]
Immunogen: Recombinant TP53 (Gly108~Lys370) expressed in E.coli. Accession No.: RPA928Hu01
[ APPLICATIONS ]
Western blotting: 0.5-2ug/ml
Immunocytochemistry in formalin fixed cells: 5-20ug/ml
Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml
Immunohistochemistry in paraffin section: 5-20ug/ml
Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml
Optimal working dilutions must be determined by end user. [ FORMULATION ]
Form & Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol. 腫瘤蛋白p53(TP53)重組蛋白[ QUALITY CONTROL ]
Content: The quality control contains recombinant TP53 disposed in loading
buffer. Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate. 5uL per well when used in enhanced chemilumescent (ECL). Note: The quality control is specifically manufactured as the positive control. Not used for other purposes. Loading Buffer: 100mM Tris(pH6.8), 1% SDS, 150mM NaCl, 50% glycerol, 0.02% BPB, 50mM DTT and 0.02% NaN3. [ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles. Store at 4
oC for frequent use. Aliquot and store at -20
oC for 12 months. Stability Test: The thermal stability is described by the loss rate. The loss rate
was determined by accelerated thermal degradation test, that is, incubate the
protein at 37
oC for 48h, and no obvious degradation and precipitation were
observed.The loss rate is less than 5% within the expiration date under
appropriate storage condition. [ IDENTIFICATION ]
A B
Figure 1. Western Blot
A. Sample: Recombinant TP53, Human B. Human MCF7 Cells Primary Ab: 2μg/mL Rabbit Anti-Human TP53 Ab
Second Ab: 1:2000 Dilution of HRP-Linked Guinea pig
Anti-Rabbit Ab (Catalog: SAA544Rb59)腫瘤蛋白p53(TP53)重組蛋白
