來源多抗制備
宿主兔
效價-
Ig類型IgG
純化方式抗原特異性親和純化
標記物無標記物
**原信號素3F(SEMA3F)多克隆抗體-
緩沖液成份磷酸鹽緩沖液（pH7.4,含有0.02% NaN3和50%甘油）
性狀液體
濃度200ug/ml
且適物種-
應用信號素3F(SEMA3F)多克隆抗體WB, ICC, IHC-P, IHC-F, ELISA
Polyclonal Antibody to Semaphorin 3F (SEMA3F) Organism Species: Homo sapiens (Human) Instruction manual FOR IN VITRO USE AND RESEARCH USE ONLY NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES 10th Edition (Revised in Jan, 2014) [ PRODUCT INFORMATION ] Immunogen: SEMA3F, Human Clonality: Polyclonal Host: Rabbit Immunoglobulin Type: IgG Purification: Affinity Chromatography. Applications: WB, ICC, IHC-P, IHC-F, ELISA Concentration: 200μg/mL UOM: 100μg [ IMMUNOGEN INFORMATION ] Immunogen: Recombinant SEMA3F (Val350~Leu483) with N-terminal His-Tag expressed in E.coli. Accession No.: RPL930Hu01 [ ANTIBODY SPECIFITY ] The antibody is a rabbit polyclonal antibody raised against SEMA3F. It has been selected for its ability to recognize SEMA3F in immunohistochemical staining and western blotting. 信號素3F(SEMA3F)多克隆抗體[ APPLICATIONS ] Western blotting: 1:50-400 Immunocytochemistry in formalin fixed cells: 1:50-500 Immunohistochemistry in formalin fixed frozen section: 1:50-500 Immunohistochemistry in paraffin section: 1:10-100 Enzyme-linked Immunosorbent Assay: 1:100-200 Optimal working dilutions must be determined by end user. [ CONTENTS ] Form & Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol. [ QUALITY CONTROL ] Content: The quality control contains recombinant SEMA3F (Val350~Leu483) disposed in loading buffer. Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate. 5uL per well when used in enhanced chemilumescent (ECL). Note: The quality control is specifically manufactured as the positive control. Not used for other purposes. Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol, BPB 0.01%, NaN3 0.02%. 信號素3F(SEMA3F)多克隆抗體[ STORAGE ] Store at 4oC for frequent use. Stored at -20oC to -80oC in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles. [ IMAGES ] Used in Western Blot, Sample: Recombinant SEMA3F, Human Used in DAB staining on fromalin fixed paraffin- embedded breast tissue

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