激肽釋放酶8(KLK8)多克隆抗體Polyclonal Antibody to Kallikrein 8 (KLK8) Organism Species: Homo sapiens (Human) Instruction manual FOR IN VITRO USE AND RESEARCH USE ONLY NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES 10th Edition (Revised in Jan, 2014) [ PRODUCT INFORMATION ] Immunogen: KLK8, Human Clonality: Polyclonal Host: Rabbit Immunoglobulin Type: IgG Purification: Affinity Chromatography. Applications: WB, ICC, IHC-P, IHC-F, ELISA Concentration: 200μg/mL UOM: 100μg [ IMMUNOGEN INFORMATION ] Immunogen: Recombinant KLK8 (Gln29~Gly260) with two N-terminal Tags, His-tag and T7-tag expressed in E.coli. Accession No.: RPA690Hu01 [ ANTIBODY SPECIFITY ] The antibody is a rabbit polyclonal antibody raised against KLK8. It has been selected for its ability to recognize KLK8 in immunohistochemical staining and western blotting. [ APPLICATIONS ] Western blotting: 1:50-400 Immunocytochemistry in formalin fixed cells: 1:50-500 Immunohistochemistry in formalin fixed frozen section: 1:50-500 Immunohistochemistry in paraffin section: 1:10-100 Enzyme-linked Immunosorbent Assay: 1:100-5000 Optimal working dilutions must be determined by end user. [ CONTENTS ] Form & Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol. [ QUALITY CONTROL ] Content: The quality control contains recombinant KLK8 (Gln29~Gly260) disposed in loading buffer. Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate. 5uL pe r well when used in enhan ced chemilumes cent (ECL ). Note: The quality control is specifically manufactured as the positive control. Not used for other purposes. Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol, BPB 0.01%, NaN3 0.02%.激肽釋放酶8(KLK8)多克隆抗體 [ STORAGE ] Store at 4 oC for frequent use. Stored at -20oC in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles. [ IMAGES ] Used in Western Blot, Sample: Recombinant KLK8, Human Used in DAB staining on fromalin fixed paraffin- embedded pancreas tissue PAA690Hu01 Polyclonal Antibody to Kallikrein 8 (KLK8) Organism Species: Homo sapiens (Human) Instruction manual FOR IN VITRO USE AND RESEARCH USE ONLY NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES 10th Edition (Revised in Jan, 2014) Used in Western Blot, Sample: Mouse Ear Tissue Primary Ab: 1μg/mL Rabbit Anti-Human KLK8 Ab Second Ab: 1:1000 Dilution of HRP-Linked Guinea pig Anti-Rabbit Ab (Catalog: SECab05)激肽釋放酶8(KLK8)多克隆抗體

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