補體因子P(CFP)多克隆抗體Polyclonal Antibody to Complement Factor P (CFP) Organism Species: Homo sapiens (Human) Instruction manual FOR IN VITRO USE AND RESEARCH USE ONLY NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES 9th Edition (Revised in Jul, 2013) [ PRODUCT INFORMATION ] Immunogen: CFP, Human Clonality: Polyclonal Host: Rabbit Immunoglobulin Type: IgG Purification: Affinity Chromatography. Applications: WB, ICC, IHC-P, IHC-F, ELISA Concentration: 200μg/mL UOM: 100μg [ IMMUNOGEN INFORMATION ] Immunogen: Recombinant CFP (Asp315~Leu469) expressed in E.coli. Accession No.: RPA783Hu01 Sequence: The target protein is fused with N-terminal His-Tag and its sequence is listed below. MG H HH HH HS GSE F -D GEW DS WGEWSP CI R R NMKSI S C QEI PGQ QS R G RT C RGRKF DGHRC AGQQQDI RHC YSIQHCP LKG SWSEWSTWG L CMPPCGPNPT RARQRLCTPL LPKYPPTVSM VEGQGEKNVT FWGRPLPRCE ELQGQKLVVE EKRPCLHVPA CKDPEEEEL [ ANTIBODY SPECIFITY ] The antibody is a rabbit polyclonal antibody raised against CFP. It has been selected for its ability to recognize CFP in immunohistochemical staining and western blotting. 補體因子P(CFP)多克隆抗體[ APPLICATIONS ] Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200 Optimal working dilutions must be determined by end user. [ IMAGES ] [ CONTENTS ] Form & Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol. [ QUALITY CONTROL ] Content: The quality control contains recombinant CFP (Asp315~Leu469) disposed in loading buffer. Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate. 5uL pe r well when used in enhan ced chemilumes cent (ECL ). Note: The quality control is specifically manufactured as the positive control. Not used for other purposes. Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol, BPB 0.01%, NaN3 0.02%.補體因子P(CFP)多克隆抗體[ STORAGE ] Store at 4 oC for frequent use. Stored at -20oC to -80 oC in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles. Used in DAB staining on fromalin fixed paraffin- embedded Spleen tissue Used in Western Blot ,Sample: Recombinant CFP,Human

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