年限： *****
細胞形態： 上皮樣
ATCC Number： CCL-42?
生長狀態： 貼壁生長
運輸方式： 凍存運輸
數量： 大量
是否是腫瘤細胞： 0
物種來源： 其他
組織來源： connective tissue and muscle
Designations: FHM
FHM細胞Depositors: RG Malsberger
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Pimephales promelas
Morphology: epithelial
Source: Tissue: connective tissue and muscle
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Isolation: Isolation date: April, 1962
Virus Resistance: poliovirus 1
Age: *****
Comments: The FHMFHM細胞 (Fat Head Minnow) line of epithelial cells was derived from tissue posterior to the anus, exclusive of the caudal fin, of normal ***** minnows of both sexes by M. Gravell and R.G. Malsberger, in April, 1962. [22174]
The cells multiply over a wide range of temperatures (0 to 36C) with optimal growth between 28C and 34C.
Propagation: ATCC complete growth medium: Minimum essential medium (Eagle) in Hanks' BSS, 90%; fetal bovine serum, 10%
Atmosphere: air, 100%
Temperature: 34.0°C; (Max. 34.0 °C, Min. 28.0°C)
Subculturing: Protocol: Remove old medium, add fresh 0.25% trypsin solution, rinse and remove. Add 1 to 2ml of trypsin solution, allow the flask to sit at room temperature until the cells detach. FHM細胞Add fresh medium, aspirate and dispense into new flasks.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended
Medium Renewal: At the time of subcultivation (this will vary depending upon the temperature of incubation)
Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
References: 22174: Gravell M, Malsberger RG. A permanent cell line from the fathead minnow (Pimephales promelas). Ann. N.Y. Acad. Sci. 126: 555-565, 1965. PubMed: 5220177
26198: Leibovitz A. FHM細胞The growth and maintenance of tissue-cell cultures in free gas exchange with the atmosphere. Am. J. Hyg. 78: 173-180, 1963. PubMed: 14063721
