細(xì)胞類(lèi)型： T**細(xì)胞
數(shù)量： 大量
是否是腫瘤細(xì)胞： 0
物種來(lái)源： 人
運(yùn)輸方式： 凍存運(yùn)輸
生長(zhǎng)狀態(tài)： 懸浮生長(zhǎng)
細(xì)胞形態(tài)： **樣
ATCC Number： CRL-8066?
相關(guān)**： 其他**
Designations: Mo [Mo T]
Mo [Mo T]細(xì)胞Depositors: Genetics Institute, Inc., Regents of the University of California
Biosafety Level: 2 [Cells Contain RETROVIRUS ]
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Homo sapiens
Morphology: lymphoblast
Source: Disease: hairy cell leukemia
Cell Type: T lymphocyte;
Cellular Products: granulocyte macrophage colony stimulating factor (GM-CSF, GM CSF), also known as neutrophil migration inhibitory factor (NIF-T); interferon
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Mo [Mo T]細(xì)胞DNA Profile (STR): Amelogenin: X,Y
CSF1PO: 10
D13S317: 9,13
D16S539: 11
D5S818: 11,12
D7S820: 9,12
THO1: 9.3
TPOX: 8,11
vWA: 17,18
Gender: male
Comments: A plasmid containing the DNA sequence coding from Mo that codes for CSF is available as ATCC 39754.
Mo cells contain a replication competent genome of Human T Cell Leukemia Virus II (HTLV-II) and two defective HTLV-II genomes.
For long term culture, the cells can be grown in medium with 2% fetal bovine serum.
Propagation: ATCC complete growth medium: Mo [Mo T]細(xì)胞The base medium for this cell line is ATCC-formulated Iscove's Modified Dulbecco's Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%.
Temperature: 37.0°C
Atmosphere: 8 to 9% CO2 in air
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:6 is recommended
The cells are subcultured every 7 days by centrifugation and division of the cell pellet 1:4 (1:6 maximum).
Do not dilute below 2 X 105 viable cells/ml or allow to exceed 2 X 106 cells/ml.
Culture at 8 to 9% CO2 in air.
The donor suggests using Falcon flasks for optimum growth.
Preservation: Freeze medium: culture medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Related Products: Recommended medium (without the additional serum described under ATCC Medium): ATCC 30-2005
Mo [Mo T]細(xì)胞Recommended serum: ATCC 30-2020
Cell culture tested DMSO: ATCC 4-X
Erythrosin B vital stain solution: ATCC 30-2404
References: 3527: Golde DW, Quan SG. Unique T-lymphocyte line and products derived therefrom. US Patent 4,438,032 dated Mar 20 1984
22772: Clark SC, et al. Method for identification and isolation of DNA encoding a desired protein. US Patent 4,675,285 dated Jun 23 1987
22828: Saxon A, et al. Immunologic characterization of hairy cell leukemias in continuous culture. J. Immunol. 120: 777-782, 1978. PubMed: 344797
23502: Gasson JC, et al. Purified human granulocyte-macrophage colony-stimulating factor: direct action on neutrophils. Science 226: 1339-1342, 1984. PubMed: 6390681
