細胞類型： B**細胞

生長狀態： 懸浮生長

運輸方式： 凍存運輸

細胞形態： **樣

是否是腫瘤細胞： 0

物種來源： 小鼠

**類型： IgA; lambda 2 light chain

品系： BALB/c

數量： 大量

ATCC Number： TIB-23?

MOPC 315細胞相關**： 多發性骨髓瘤

Designations: MOPC 315

Depositors: M Cohn

Isotype: IgA; lambda 2 light chain

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Mus musculus

Morphology: lymphoblast

Source: Disease: plasmacytoma; myeloma

Strain: BALB/c

Cell Type: B lymphocyte;

MOPC 315細胞Cellular Products: immunoglobulin; monoclonal antibody; against DNP and TNP substituted proteins

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Antigen Expression: H-2d

Comments: This line was established from the MOPC 315 plasmacytoma (induced with mineral oil in a BALB/c mouse).

The cells are killed by 10 nM dexamethasone.

Initial recovery from the frozen state is slow, and approximately 7 to 10 days are required before any noticeable cell growth occurs.

Tested and found negative for ectromelia virus (mousepox).

Propagation: ATCC complete growth medium: MOPC 315細胞The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Subculturing: Medium Renewal: Every 2 to 3 days

Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.

References: 1114: Gehring U, et al. Glucocorticoid action on hybrid clones derived from cultured myeloma and lymphoma cell lines. Proc. Natl. Acad. Sci. USA 69: 3124-3127, 1972. PubMed: 4343956

22228: Halpern MS, Coffman RL. Polymer formation and J chain synthesis in mouse plasmacytomas. J. Immunol. 109: 674-680, 1972. PubMed: 5074326

23163: Potter M. Immunoglobulin-producing tumors and myeloma proteins of mice. Physiol. MOPC 315細胞Rev. 52: 631-719, 1972. PubMed: 4555516