LLC-PK1細胞
器官來源： 腎臟
數(shù)量： 大量
細胞形態(tài)： 上皮樣
品系： Hampshire
年限： 3 to 4 weeks
是否是腫瘤細胞： 0
物種來源： 豬
運輸方式： 凍存運輸
ATCC Number： CL-101?
相關(guān)**： 正常
Designations: LLC-PK1
Depositors: Eli Lilly & Co.
LLC-PK1細胞Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Organism: Sus scrofa
Morphology: epithelial
Source: Organ: kidney
Strain: Hampshire
Disease: normal
Cellular Products: plasminogen activator
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Age: 3 to 4 weeks
Gender: male
Propagation: ATCC complete growth medium: The base medium for this cell line is Medium 199 containing 1.5 g/L to 2.2 g/L sodium bicarbonate. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 3%.
Temperature: 37.0°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:8 is recommended
Medium Renewal: Twice per week
LLC-PK1細胞Protocol: Volumes used in this protocol are for 75 sq cm flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with Ca++/Mg++ free Dulbecco's phosphate-buffered saline (D-PBS) or 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C to facilitate dispersal.
Add 2.0 to 3.0 ml of complete growth medium and aspirate cells by gently pipetting
Resuspend the cell pellet in fresh growth medium. Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37C.
Preservation: culture medium 95%; DMSO, 5%
Related Products:LLC-PK1細胞 recommended serum:ATCC 30-2020
formerly distributed as:ATCC CRL-1392
References: 3520: Hull RN, Huseby RM. Enhanced production of plasminogen activator. US Patent 3,904,480 dated Sep 9 1975
22659: Perantoni A, Berman JJ. Properties of Wilms' tumor line (TuWi) and pig kidney line (LLC-PK1) typical of normal kidney tubular epithelium. In Vitro 15: 446-454, 1979. PubMed: 225262
28301: Loffler S, et al. CD9, a tetraspan transmembrane protein, LLC-PK1細胞renders cells susceptible to canine distemper virus. J. Virol. 71: 42-49, 1997. PubMed: 8985321
