NRK-52E細胞 
	
		生長狀態:	貼壁生長
	
	
		運輸方式:	凍存運輸
	
	
		細胞形態:	上皮樣
	
	
		是否是腫瘤細胞:	0
	
	
		物種來源:	大鼠
	
	
		ATCC Number:	CRL-1571?
	
	
		數量:	大量
	
	
		器官來源:	腎臟
	
	
		相關**:	正常
	
	
		Designations:	NRK-52E
	
	
		Depositors:	 JE DeLarco
	
	
		Biosafety Level:	1
	
	
		Shipped:	frozen
	
	
		Medium & Serum:	See Propagation
	
	
		NRK-52E細胞Growth Properties:	adherent
	
	
		Organism:	Rattus norvegicus deposited as Rattus sp.
	
	
		Morphology:	epithelial
	
	
		
	
	
		Source:	Organ: kidney
	
	
		Disease: normal
	
	
		Permits/Forms:	In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. NRK-52E細胞Please click here for information regarding the specific requirements for shipment to your location.
	
	
		Applications:	transfection host
	
	
		Receptors:	epidermal growth factor (EGF); multiplication stimulating activity (MSA)
	
	
		Propagation:	ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose, 95%; bovine calf serum, 5%
	
	
		Temperature: 37.0°C
	
	
		Atmosphere: air, 95%; carbon dioxide (CO2), 5%
	
	
		Subculturing:	Protocol: Remove medium, and rinse with 0.25% trypsin, 0.02% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. NRK-52E細胞Allow the flask to sit at room temperature (or at 37C) until the cells detach. Centrifuge the cell suspension at 1000 rpm for 10 minutes, resuspend the pellet in fresh culture medium, aspirate and dispense into new culture vessels.
	
	
		Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:4 is recommended
	
	
		Medium Renewal: 2 to 3 times per week
	
	
		Preservation:	Freeze medium: Complete growth medium 95%; DMSO, 5%
	
	
		Storage temperature: liquid nitrogen vapor temperature
	
	
		Related Products:	Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
	
	
		recommended serum:ATCC 30-2030
	
	
		References:	22713: de Larco JE, Todaro GJ. Epithelioid and fibroblastic rat kidney cell clones: epidermal growth factor (EGF) receptors and the effect of mouse sarcoma virus transformation.NRK-52E細胞 J. Cell. Physiol. 94: 335-342, 1978. PubMed: 304450
	
	
		23197: Huu D, et al. Persistent infection of a rat kidney cell line with Rauscher murine leukemia virus. J. Bacteriol. 92: 1133-1140, 1966. PubMed: 5926742