RS4;11細胞
年限： 32 years
是否是腫瘤細胞： 0
物種來源： 人
數(shù)量： 大量
生長狀態(tài)： 懸浮生長
ATCC Number： CRL-1873?
相關**： 白血病
細胞形態(tài)： **樣
運輸方式： 凍存運輸
器官來源： 骨髓
Designations: RS4;11
Depositors: JH Kersey
RS4;11細胞Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Homo sapiens
Morphology: lymphoblast
Source: Organ: bone marrow
Disease: acute lymphoblastic leukemia
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Antigen Expression: HLA DR +; CD9 +; CD24 +
DNA Profile (STR): Amelogenin: X
CSF1PO: 11,12
D13S317: 11
D16S539: 11,12
RS4;11細胞D5S818: 12,13
D7S820: 8,12
THO1: 6,9.3
TPOX: 8
vWA: 14,17
Cytogenetic Analysis: The cells have a characteristic chromosomal abnormality, t(4;11)(q21;q23), and an isochromosome for the long arm of chromosome 7.
Age: 32 years
Gender: female
Ethnicity: Caucasian
Comments: This line was established from the bone marrow of a patient with acute lymphoblastic leukemia.The cells lack surface and cytoplasmic immunoglobulin, and are negative for CALLA (CD10). The cells are also negative for myeloperoxidase and chloroacetate esterase and do not stain with Sudan Black.The cells are strongly positive for terminal deoxynucleotidyl transferase (TdT) and are positive for BA-1 (CD24), BA-2 (CD9) and MHC Class II antigens (HLA DR).
Propagation: ATCC complete growth medium: RS4;11細胞The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Subculturing: Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 X 10 exp5 viable cells/ml. Maintain cell density between 1 X 10 exp5 and 1 X 10 exp6 viable cells/ml.
Medium Renewal: Every 2 to 3 days
Preservation:RS4;11細胞 Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor temperature
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
recommended serum:ATCC 30-2020
Cell culture tested DMSO:ATCC 4-X
References: 22302: Stong RC, et al. Human acute leukemia cell line with the t(4;11) chromosomal rearrangement exhibits B lineage and monocytic characteristics. Blood 65: 21-31, 1985. PubMed: 3917311
