JC細胞
年限： 1.5 years
細胞形態： 上皮樣
數量： 大量
運輸方式： 凍存運輸
器官來源： 乳腺
是否是腫瘤細胞： 0
物種來源： 小鼠
品系： BALB/cRos
生長狀態： 貼壁生長
ATCC Number： CRL-2116?
相關**： 腺癌
Designations: JC
Depositors: TM Chu
JC細胞Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Mus musculus
Morphology: epithelial
Source: Organ: mammary gland
Strain: BALB/cRos
Disease: adenocarcinoma
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. JC細胞Please click here for information regarding the specific requirements for shipment to your location.
Tumorigenic: Yes
Cytogenetic Analysis: modal number = 40; range = 26 to 100
Age: 1.5 years
Gender: female
JC細胞Comments: JC is an epithelial-like cell line established in 1983 from a spontaneous primary mammary adenocarcinoma along the milk line.
The original tumor was passaged once in female BALB/c mice, and the resulting tumors were harvested and passaged in vitro for six months.
Electron microscopic examination detected the presence of cellular tonofilaments and desmosomes.
No type B or C virus particles were detected, although intracisternal A particles were observed occasionally.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:6 is recommended
Medium Renewal: Twice per week
Remove medium, rinse with 0.25% trypsin, 0.03% EDTA solution and remove trypsin. Add fresh trypsin solution and let the culture sit at room temperature (or at 37C) until the cells detach. JC細胞Add fresh medium, aspirate and dispense into new flasks.
Preservation: Culture medium, 95%; DMSO, 5%
Doubling Time: 12 to 16 hrs
References: 22452: Capone PM, et al. Immunotherapy in a spontaneously developed murine mammary carcinoma with syngeneic monoclonal antibody. Cancer Immunol. Immunother. 25: 93-99, 1987. PubMed: 3664534
22679: Chao TY, Chu TM. Characterization of a new spontaneously developed murine mammary adenocarcinoma in syngeneic BALB/c hosts. In Vitro Cell. Dev. Biol. 25: 621-626, 1989. PubMed: 2666384
