MDOK細胞
數(shù)量： 大量
是否是腫瘤細胞： 0
物種來源： 綿羊
器官來源： 腎臟
ATCC Number： CRL-1633?
運輸方式： 凍存運輸
相關**： 正常
細胞形態(tài)： 上皮樣
生長狀態(tài)： 貼壁生長
Designations: MDOK
Depositors: RB Owens
Biosafety Level: 1
Shipped: frozen
MDOK細胞Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Ovis aries
Morphology: epithelial
Source: Organ: kidney
Disease: normal
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Virus Susceptibility: MDOK細胞Vesicular stomatitis (Indiana and New Jersey); infectious bovine rhinotracheitis; sheep bluetongue virus
Gender: male
Propagation: ATCC complete growth medium: Minimum essential medium (Eagle) with non-essential amino acids and sodium pyruvate, 90%; fetal bovine serum, 10%
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
Remove medium, rinse with fresh 0.25% trypsin solution, remove trypsin and let the culture sit at room temperature (or at 37C) until the cells detach MDOK細胞(about 10 minutes). Add fresh medium, aspirate and dispense into new flasks.
References: 22202: Nelson-Rees WA, et al. Debut and accumulation of centric fusion products: an index to age of certain cell lines. Cytogenetics 6: 436-450, 1967. PubMed: 5587551
22217: Madin SH, Darby NB Jr.. Established kidney cell lines of normal ***** bovine and ovine origin. Proc. Soc. Exp. Biol. Med. 98: 574-576, 1958. PubMed: 13567776
26306: . . Chromosoma 16: 79-89, 1965.
