32D Clone 3細(xì)胞
是否是腫瘤細(xì)胞： 0
物種來源： 小鼠
ATCC Number： CRL-11346?
運(yùn)輸方式： 凍存運(yùn)輸
數(shù)量： 大量
器官來源： 骨髓
生長狀態(tài)： 混合型生長
細(xì)胞形態(tài)： **樣
Designations: 32D Clone 3
Depositors: Amgen, Inc.
Biosafety Level: 1
Shipped: frozen
32D Clone 3細(xì)胞Medium & Serum: See Propagation
Growth Properties: mixed, adherent and suspension
Organism: Mus musculus
Morphology: lymphoblast
Source: Organ: bone marrow
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Applications: transfection host
Comments: This line is IL-3 dependent.
The line was originally contaminated with mycoplasma, and was cured by treatment with BM-cycline.
The cells were assayed for mycoplasma, by the Hoechst stain, PCR and the standard culture test, after a six-week period following treatment. All tests were negative.
Propagation: 32D Clone 3細(xì)胞ATCC complete growth medium: RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate supplemented with:10% heat-inactivated fetal bovine serum10% mouse Interleukin-3 culture supplement (available from Becton Dickinson Cat. No. 354040, formerly Collaborative Research Cat. No. 40040)
Temperature: 37.0°C
Subculturing: Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 X 10 exp5 viable cells/ml. Maintain cell density between 2 X 10 exp5 and 1 X 10 exp6 viable cells/ml. 32D Clone 3細(xì)胞Scrape off the attached cells and transfer along with the floating cells into new flasks.
Medium Renewal: Every 2 to 3 days
Preservation: Freeze medium: Complete growth medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor temperature
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
References: 49723: Ahmed N, Berridge MV. Regulation of glucose transport by interleukin-3 in growth factor-dependent and oncogene-transformed bone marrow-derived cell lines. Leuk. Res. 21: 609-618, 1997. PubMed: 9301681
50750: Ahmed N, Berridge MV. 32D Clone 3細(xì)胞Distinct regulation of glucose transport by interleukin-3 and oncogenes in a murine bone marrow-derived cell line. Biochem. Pharmacol. 57: 387-396, 1999. PubMed: 9933027
