RF/6A細胞 
	
		年限:	fetus
	
	
		數量:	大量
	
	
		ATCC Number:	CRL-1780?
	
	
		相關**:	正常
	
	
		細胞形態:	上皮樣
	
	
		運輸方式:	凍存運輸
	
	
		生長狀態:	貼壁生長
	
	
		細胞類型:	內皮細胞
	
	
		是否是腫瘤細胞:	0
	
	
		物種來源:	獼猴
	
	
		組織來源:	choroid; retina
	
	
		Designations:	RF/6A
	
	
		RF/6A細胞Depositors:	 F Hu
	
	
		Biosafety Level:	1
	
	
		Shipped:	frozen
	
	
		Medium & Serum:	See Propagation
	
	
		Growth Properties:	adherent
	
	
		Organism:	Macaca mulatta
	
	
		Morphology:	epithelial
	
	
		
	
	
		Source:	Tissue: choroid; retina
	
	
		Disease: normal
	
	
		Cell Type: endothelial
	
	
		Cellular Products:	factor VIII; fibronectin
	
	
		Permits/Forms:	In addition to the MTA mentioned above, RF/6A細胞other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
	
	
		Applications:	Late passage cells have been used as feeder layers to enhance attachment, subcultivation and maintenance of uveal melanocytes.
	
	
		Age:	fetus
	
	
		Comments:	This line spontaneously transformed at an early age and has been passaged over 540 times.
	
	
		Weibel-Palade granules are seen by electron microscopy.
	
	
		Late passage cells have been used as feeder layers to enhance attachment, subcultivation and maintenance of uveal melanocytes.
	
	
		Propagation:	ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
	
	
		Temperature: 37.0°C
	
	
		Atmosphere: air, 95%; carbon dioxide (CO2), 5%
	
	
		Subculturing: RF/6A細胞Protocol: Remove medium, add fresh 0.25% trypsin, 0.53 mM EDTA solution for 2 minutes at room temperature, remove trypsin and let the culture sit at 37C for 5 minutes (or until the cells detach). Add fresh medium, aspirate and dispense into new flasks.
	
	
		Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
	
	
		Medium Renewal: Twice weekly
	
	
		Preservation:	Freeze medium: Complete growth medium, 95%; DMSO, 5%
	
	
		Storage temperature: liquid nitrogen vapor temperature
	
	
		Related Products:	Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003
	
	
		recommended serum:ATCC 30-2020
	
	
		References:	22315: Lou DA, Hu FN. RF/6A細胞Specific antigen and organelle expression of a long-term rhesus endothelial cell line. In Vitro Cell. Dev. Biol. 23: 75-85, 1987. PubMed: 3102454