293 c18細(xì)胞
運(yùn)輸方式： 凍存運(yùn)輸
年限： fetus
細(xì)胞類型： 其他細(xì)胞類型
是否是腫瘤細(xì)胞： 0
物種來源： 人
生長(zhǎng)狀態(tài)： 貼壁生長(zhǎng)
細(xì)胞形態(tài)： 上皮樣
數(shù)量： 大量
器官來源： 腎臟
ATCC Number： CRL-10852?
Designations: 293 c18
293 c18細(xì)胞Depositors: Stanford University
Biosafety Level: 2 [CELLS CONTAIN ADENOVIRUS ]
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens
Morphology: epithelial
Source: Organ: kidney
Cell Type: transformed with adenovirus 5 DNA
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
DNA Profile (STR): Amelogenin: X
CSF1PO: 11,12
D13S317: 12,14
D16S539: 9
D5S818: 8,9
293 c18細(xì)胞D7S820: 11,12
THO1: 7,9.3
TPOX: 11
vWA: 16,19
Age: fetus
Comments: This line is a derivative of 293 (see ATCC CRL-1573) the cells express EBNA1.
Very high frequencies of transformation are obtained with vectors containing the Epstein-Barr virus (EBV) oriP.
Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose supplemented with 0.25 mg/ml G-418, 90%; fetal bovine serum, 10%
Temperature: 37.0°C
Subculturing: Protocol: Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. 293 c18細(xì)胞Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:10 is recommended
Medium Renewal: Twice per week
Preservation: Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor temperature
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2020
293 c18細(xì)胞parental cell line:ATCC CRL-1573
