B6H12.2細胞
是否是腫瘤細胞： 0
物種來源： 小鼠
ATCC Number： HB-9771?
運輸方式： 凍存運輸
數量： 大量
生長狀態： 懸浮生長
細胞形態： **樣
**類型： IgG1
Designations: B6H12.2
Depositors: Washington Univ.
Isotype: IgG1
B6H12.2細胞Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products: immunoglobulin; monoclonal antibody; against integrin associated protein (CD47) and vitonectin receptor (VnR)
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Comments: Animals were immunized with purified integrin associated protein (which is physically and functionally associated with vitronectin receptors).
B6H12.2細胞Spleen cells were fused with P3X63Ag8.653 myeloma cells.
The antibody reacts with vitronectin receptors on human polymorphonuclear leukocytes (PMN, neutrophils) and blocks the receptor binding to the tripeptide Arg-Gly-Asp (RGD).
The antibody also inhibits RGD stimulated phagocytosis.
It precipitates a heterodimer of apparent molecular weights 140000 and 105000 daltons.
The antibody does not react with gpIIb/III or the LFA-1/Mac-1 family of cellular adhesion receptors.
Propagation: ATCC complete growth medium: B6H12.2細胞Iscove's modified Dulbecco's medium, 80%; fetal bovine serum, 20%
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
References: 21982: Brown EJ. Novel monoclonal antibodies. US Patent 5,057,604 dated Oct 15 1991
23417: Lindberg FP, et al. Rh-related antigen CD47 is the signal-transducer integrin-associated protein. J. Biol. Chem. 269: 1567-1570, 1994. B6H12.2細胞PubMed: 8294396
