HEK-293細胞

生長狀態： 貼壁生長

細胞形態： 上皮樣

ATCC Number： CRL-1573?

是否是腫瘤細胞： 0

物種來源： 人

年限： fetus

器官來源： 胚胎腎

數量： 大量

運輸方式： 凍存運輸

Designations: 293 [HEK-293]

Depositors: FL Graham

HEK-293細胞Biosafety Level: 2 [CELLS CONTAIN ADENOVIRUS ]

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens

Morphology: epithelial

Source: Organ: embryonic kidney

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Restrictions: These cells are distributed for research purposes only. 293 cells, their products, or their derivatives may not be distributed to third parties.

Applications: efficacy testing

transfection host

viruscide testing

Receptors: vitronectin, expressed

Tumorigenic: YES

DNA Profile (STR): Amelogenin: X

CSF1PO: 11,12

D13S317: 12,14

D16S539: 9,13

D5S818: 8,9

HEK-293細胞D7S820: 11,12

THO1: 7,9.3

TPOX: 11

vWA: 16,19

Cytogenetic Analysis: This is a hypotriploid human cell line. The modal chromosome number was 64, occurring in 30% of cells. The rate of cells with higher ploidies was 4.2 %. The der(1)t(1;15) (q42;q13), der(19)t(3;19) (q12;q13), der(12)t(8;12) (q22;p13), and four other marker chromosomes were common to most cells. Five other markers occurred in some cells only. The marker der(1) and M8 (or Xq+) were often paired. There were four copies of N17 and N22. Noticeably in addition to three copies of X chromosomes, there were paired Xq+, and a single Xp+ in most cells.

Age: fetus

Comments: Although an earlier report suggested that the cells contained Adenovirus 5 DNA from both the right and left ends of the viral genome [RF32764], it is now clear that only left end sequences are present.

The line is excellent for titrating human adenoviruses.

The cells express an unusual cell surface receptor for vitronectin composed of the integrin beta-1 subunit and the vitronectin receptor alpha-v subunit.

The Ad5 insert was cloned and sequenced, and it was determined that a colinear seqment from nts 1 to 4344 is integrated into chromosome 19 (19q13.2).

Propagation: HEK-293細胞ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 37.0°C

The cell line does not adhere to the substrate when left at room temperature for any length of time, therefore, live cultures may be received with the cells detached.The cells will re-attach to the flask over a period of several days in culture at 37C.

Subculturing: Protocol:

Remove and discard culture medium.

Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.

Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37?C to facilitate dispersal.

Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

Add appropriate aliquots of the cell suspension to new culture vessels.An inoculum of 2 X 10 (3) to 6 X 10 (3) viable cells/cm2 is recommended.

Incubate cultures at 37?C.6. Subculture when cell concentration is between 6 and 7 X 10(4) cells/cm2.

Subcultivation Ratio: 1:6 to 1:10 weekly

HEK-293細胞Medium Renewal: Every 2 to 3 days

Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO

Storage temperature: liquid nitrogen vapor phase

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium): ATCC 30-2003

derivative: ATCC CRL-10852

derivative: ATCC CRL-12006

derivative: ATCC CRL-12007

derivative: ATCC CRL-12013

derivative: ATCC CRL-12479

derivative: ATCC CRL-2029

derivative: ATCC CRL-2368

purified DNA:ATCC CRL-1573D

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92579: Standard Test Method for Determining the Virus-Eliminating Effectiveness of Liquid Hygienic Handwash and Handrub Agents Using the Fingerpads of Adult Volunteers. West Conshohocken, PA:ASTM International;ASTM Standard Test Method E 1838-02.

92587: Standard Quantitative Disk Carrier Test Method for Determining the Bactericidal, Virucidal, Fungicidal, Mycobactericidal and Sporicidal Activities of Liquid Chemical Germicides. West Conshohocken, PA:ASTM International;ASTM Standard Test Method E 2197-02.