M3WT5細(xì)胞
數(shù)量： 大量
是否是腫瘤細(xì)胞： 0
物種來源： 倉鼠
器官來源： 卵巢
運(yùn)輸方式： 凍存運(yùn)輸
細(xì)胞形態(tài)： 上皮樣
ATCC Number： CRL-1982?
生長(zhǎng)狀態(tài)： 貼壁生長(zhǎng)
Designations: M3WT5
Depositors: CM Fraser
Biosafety Level: 1
M3WT5細(xì)胞Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Cricetulus griseus
Morphology: epithelial
Source: Organ: ovary
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Receptors: acetylcholine, muscarinic m3
Gender: female
Comments: M3WT5細(xì)胞The M3WT5 cell line was derived by transfecting CHO-K1 cells (ATCC CCL-61) with the pSVL expression vector containing the gene for the rat m3 muscarinic acetylcholine receptor.
The resulting cells were co-transfected with pMSVNeo and selected for growth in medium containing 0.5 mg/ml G418.
M3WT5 cells are reported to express 690 fmol of receptor protein per mg of membrane protein.
Propagation: ATCC complete growth medium: Ham's F12 medium with 0.05 to 0.1 mg/ml G418, 90%; fetal bovine serum, 10%
Temperature: 37.0°C
Subculturing: Protocol: Remove medium, add fresh 0.25% trypsin, 0.03% EDTA solution, rinse and remove trypsin. Allow the flask to sit at room temperature (or incubate at 37C) until the cells detach (usually 5 to 10 minutes).
Subcultivation Ratio: M3WT5細(xì)胞A subcultivation ratio of 1:4 to 1:8 is recommended
Medium Renewal: At the time of subcultivation
Preservation: Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Related Products: recommended serum:ATCC 30-2020
References: 22692: Buck MA, Fraser CM. Muscarinic acetylcholine receptor subtypes which selectively couple to phospholipase C: pharmacological and biochemical properties. Biochem. Biophys. Res. Commun. M3WT5細(xì)胞173: 666-672, 1990. PubMed: 2175603
