H-4-II-E細(xì)胞
細(xì)胞形態(tài)： 上皮樣
是否是腫瘤細(xì)胞： 0
物種來(lái)源： 大鼠
ATCC Number： CRL-1548?
器官來(lái)源： 肝
相關(guān)**： 肝癌
數(shù)量： 大量
運(yùn)輸方式： 凍存運(yùn)輸
生長(zhǎng)狀態(tài)： 貼壁生長(zhǎng)
Designations: H-4-II-E
Depositors: JA Bradlaw
H-4-II-E細(xì)胞Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Rattus norvegicus deposited as Rattus sp.
Morphology: epithelial
Source: Organ: liver
Disease: hepatoma
Cellular Products: aryl hydrocarbon hydroxylase is inducible
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
H-4-II-E細(xì)胞Comments: The line has been cured of Mycoplasma arginini contamination.
The line is useful for detecting picogram quantities of planar polychlorinated organic compounds in environmental samples and food extracts.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: Every 2 to 3 days
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Preservation: culture medium 95%; DMSO, 5%
Related Products: Recommended mediumH-4-II-E細(xì)胞 (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003
recommended serum:ATCC 30-2020
References: 4196: Benedict WF, et al. Aryl hydrocarbon hydroxylase induction in mammalian cell culture--IV. Stimulation of the enzyme activity in established cell lines derived from rat or mouse hepatoma and from normal rat liver. Biochem. Pharmacol. 22: 2766-2769, 1973. PubMed: 4357894
22186: . . In Vitro 16: 230, 1980.
23140: Sassier P, Bergeron M. Specific inhibition of cell proliferation in the mouse intestine by an aqueous extract of rabbit small intestine. Cell Tissue Kinet. 10: 223-243, 1977. PubMed: 872181
58038: Bradlaw JA, Casterline JL Jr.. Induction of enzyme activity in cell culture: a rapid screen for detection of planar polychlorinated organic compounds. J. Assoc. Off. Anal. Chem. 62: 904-916, 1979. PubMed: 115836
58076: Peraino C, et al. H-4-II-E細(xì)胞Hepatomas in tissue culture compared with adapting liver in vivo. Natl. Cancer Inst. Monogr. 13: 229-245, 1964. PubMed: 14143233
