CRFK細胞 
	
		ATCC Number:	CCL-94?
	
	
		相關**:	正常
	
	
		數量:	大量
	
	
		是否是腫瘤細胞:	0
	
	
		物種來源:	貓
	
	
		年限:	12 weeks
	
	
		細胞形態:	上皮樣
	
	
		生長狀態:	貼壁生長
	
	
		運輸方式:	凍存運輸
	
	
		器官來源:	腎皮質
	
	
		CRFK細胞Designations:	CRFK
	
	
		Depositors:	 RA Van Deusen
	
	
		Biosafety Level:	1
	
	
		Shipped:	frozen
	
	
		Medium & Serum:	See Propagation
	
	
		Growth Properties:	adherent
	
	
		Organism:	Felis catus
	
	
		Morphology:	epithelial
	
	
		
	
	
		Source:	Organ: kidney, cortex
	
	
		Disease: normal
	
	
		Permits/Forms:	In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
	
	
		CRFK細胞Applications:	transfection host
	
	
		Age:	12 weeks
	
	
		Gender:	female
	
	
		Comments:	The cells are free of bovine viral diarrhea (BVD) virus.
	
	
		Propagation:	ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: horse serum to a final concentration of 10%.
	
	
		Temperature: 37.0°C
	
	
		Atmosphere: air, 95%; carbon dioxide (CO2), 5%
	
	
		Subculturing:	Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:8 is recommended
	
	
		Medium Renewal: 2 to 3 times per week
	
	
		Remove medium, add fresh 0.25% trypsin 0.02% EDTA solution for 1 to 2 minutes, remove trypsin and allow culture to stand at room temperature (or at 37C) until cells detach . CRFK細胞Add fresh medium, aspirate and dispense into new flasks.
	
	
		Preservation:	Freeze medium: complete growth medium, 95%; DMSO, 5%
	
	
		Related Products:	Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003
	
	
		References:	26266: Crandell RA, et al. Development, characterization, and viral susceptibility of a feline (Felis catus) renal cell line (CRFK). In Vitro 9: 176-185, 1973. PubMed: 4130570
	
	
		26267: Fabricant CG, Rich LJ. Microbial studies of feline urolithiasis. J. Am. Vet. Med. Assoc. 158: 976-980, 1971. PubMed: 4930310
	
	
		26268: Lee KM, et al. Use of an established cat cell line for investigation and quantitation of feline oncornaviruses. J. Natl. Cancer Inst. 49: 55-60, 1972. PubMed: 4338781
	
	
		CRFK細胞28301: Loffler S, et al. CD9, a tetraspan transmembrane protein, renders cells susceptible to canine distemper virus. J. Virol. 71: 42-49, 1997. PubMed: 8985321