ES-E14TG2a細(xì)胞 
	
		數(shù)量:	大量
	
	
		年限:	embryo, blastocyst embryo, blastocyst
	
	
		細(xì)胞類型:	其他細(xì)胞類型
	
	
		品系:	129/Ola
	
	
		是否是腫瘤細(xì)胞:	0
	
	
		物種來源:	小鼠
	
	
		器官來源:	胚胎
	
	
		生長狀態(tài):	貼壁生長
	
	
		ATCC Number:	CRL-1821?
	
	
		細(xì)胞形態(tài):	其他
	
	
		運輸方式:	凍存運輸
	
	
		Designations:	ES-E14TG2a
	
	
		ES-E14TG2a細(xì)胞Depositors:	 T Doetschman
	
	
		Biosafety Level:	1
	
	
		Shipped:	frozen
	
	
		Medium & Serum:	See Propagation
	
	
		Growth Properties:	adherent
	
	
		Organism:	Mus musculus
	
	
		Morphology:	spherical
	
	
		
	
	
		Source:	Organ: embryo
	
	
		Cell Type: embryonic stem cell , blastocyst derived
	
	
		Strain: 129/Ola
	
	
		Permits/Forms:	In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
	
	
		Applications:	transfection host
	
	
		Age: ES-E14TG2a細(xì)胞embryo, blastocyst embryo, blastocyst
	
	
		Comments:	This is a derivative of one of several embryonal stem cell (ES) lines developed by M. Hooper in 1987.
	
	
		The cells are deficient in HGPRT (HPRT) and are resistant to 0.06 mM 6-thioguanine.
	
	
		The cells remain undifferentiated when cultured on feeder layers (embryonic fibroblasts or STO cells).
	
	
		The cells spontaneously differentiate and form embryonal structures in the absence of a feeder layer.
	
	
		When injected into blastocysts, the cells can colonize the germline.
	
	
		After conventional molecular gene modification techniques, they may be used to reconstitute mouse embryos.
	
	
		They may also be of use in studies of early post implantation structures.
	
	
		Tested and found negative for ectromelia virus (mousepox).
	
	
		Propagation:	ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: 2-mercaptoethanol to a final concentration of 0.1 mM ; fetal bovine serum to a final concentration of 10%.
	
	
		Temperature: 37.0°C
	
	
		Atmosphere: air, 95%; carbon dioxide (CO2), 5%
	
	
		Subculturing:	Protocol: ES-E14TG2a細(xì)胞Remove medium, add fresh 0.25% trypsin, 0.53mM EDTA solution. Allow the trypsin to loosen the cells, add fresh medium, aspirate and dispense into new flasks containing pre-plated irradiated feeder cells.
	
	
		Subcultivation Ratio: A subcultivation ratio of 1:5 is recommended
	
	
		Medium Renewal: Every 2 to 3 days
	
	
		Preservation:	Freeze medium: Complete growth medium, 95%; DMSO, 5%
	
	
		Storage temperature: liquid nitrogen vapor phase
	
	
		Related Products:	Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
	
	
		recommended serum:ATCC 30-2020
	
	
		feeder layer cells:ATCC 56-X
	
	
		References:	22254: Smith AG, Hooper ML. Buffalo rat liver cells produce a diffusible activity which inhibits the differentiation of murine embryonal carcinoma and embryonic stem cells. Dev. Biol. 121: 1-9, 1987. PubMed: 3569655
	
	
		22923: Kuehn MR, et al. A potential animal model for Lesch-Nyhan syndrome through introduction of HPRT mutations into mice. Nature 326: 295-298, 1987. PubMed: 3029599
	
	
		22925: Doetschman T, et al. ES-E14TG2a細(xì)胞Targetted correction of a mutant HPRT gene in mouse embryonic stem cells. Nature 330: 576-578, 1987. PubMed: 3683574