20B8細胞
器官來源： 腎臟
ATCC Number： CRL-12582?
相關**： **瘤
細胞形態： 上皮樣
生長狀態： 貼壁生長
運輸方式： 凍存運輸
細胞類型： 其他細胞類型
數量： 大量
20B8細胞是否是腫瘤細胞： 0
物種來源： 人
Designations: 20B8
Depositors: Bayer Corporation
Biosafety Level: 2
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens
Morphology: epithelial
Source: Organ: kidney
Disease: Burkitt's lymphoma
Cell Type: 20B8細胞epithelial; somatic hybrid transfected with plasmid pSV2neo
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DNA Profile (STR): Amelogenin: X,Y
CSF1PO: 10,11,12
D13S317: 12,14
D16S539: 9
D5S818: 8,9,12
D7S820: 10,11
THO1: 7,9,9.3
TPOX: 6,11
vWA: 15,19
Comments: The 20B8 cell line was established by transfection of HKB-11 cells (ATCC CRL-12568) with pCIS25DTR expression vector coding for a B-domain deleted (BDD) FVIII. This cell line can be used for high productivity of B-domain deleted Factor VIII.
Propagation: 20B8細胞ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Subculturing: Protocol:
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37?C to facilitate dispersal.
Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37?C.
Medium Renewal: Two to three times weekly
Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Related Products: 20B8細胞Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
recommended serum: ATCC 30-2020
parental cell line: HKB-11, ATCC CRL-12568
related Cell line: 1G2, ATCC CRL-13005 (PTA-8
References: 70369: Cho MS, et al. Expression system for factor VIII. US Patent 6,358,703 dated Mar 19 2002
