HFN 7.1細胞
器官來源： 脾
細胞形態(tài)： **樣
**類型： IgG1
是否是腫瘤細胞： 0
物種來源： 小鼠
ATCC Number： CRL-1606?
數量： 大量
生長狀態(tài)： 懸浮生長
運輸方式： 凍存運輸
Designations: HFN 7.1
Depositors: RC Schoen, KL Bentley, RJ Klebe
Isotype: IgG1
HFN 7.1細胞Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Organ: spleen
Cell Type: hybridoma: B lymphocyte;
Cellular Products: immunoglobulin; monoclonal antibody; against human fibronectin
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Antigen Expression: HFN 7.1細胞H-2d
Comments: The antibody does not cross-react with other fibronectins tested (26 species including 15 primates).
Mice were immunized with purified human fibronectin.
Spleen cells were fused with P3X63Ag8 myeloma cells.
Tested and found negative for ectromelia virus (mousepox).
Formerly ATCC HB-39.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. HFN 7.1細胞To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 X 10 exp5 viable cells/ml and subculture at 1 X 10 exp6 cells/ml.
References: 57999: Schoen RC, et al. HFN 7.1細胞Monoclonal antibody against human fibronectin which inhibits cell attachment. Hybridoma 1: 99-108, 1982. PubMed: 6208125
