11F11細(xì)胞
生長(zhǎng)狀態(tài)： 懸浮生長(zhǎng)
數(shù)量： 大量
ATCC Number： CRL-1908?
運(yùn)輸方式： 凍存運(yùn)輸
是否是腫瘤細(xì)胞： 0
物種來(lái)源： 小鼠
細(xì)胞形態(tài)： **樣
**類型： IgG2a; kappa light chain
Designations: 11F11
Depositors: AD O'Brien
Isotype: IgG2a; kappa light chain
11F11細(xì)胞Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma)
Morphology: lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products: immunoglobulin; monoclonal antibody; against Shiga like toxin II (SLT-II)
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments: 11F11細(xì)胞Animals were immunized with a formalinized toxoid prepared from crude SLT-II produced by an E. coli K-12 strain lysogenized with the 933W toxin converting phage of enterohemorrhagic E. coli 933.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
The antibody reacts with the A subunit of SLT-II and does not react with B subunit.
The antibody can be used in SLT-II neutralization assays and in assays to detect SLT-II producing E. coli strains.
It may also be used to differentiate SLT-II from SLT-I.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing: 11F11細(xì)胞Medium Renewal: Every 2 to 4 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 x 10 exp5 cells/ml and maintain between 1 x 10 exp5 and 1 x 10 exp6 cells/ml.
References: 1784: Perera LP, et al. Isolation and characterization of monoclonal antibodies to Shiga-like toxin II of enterohemorrhagic Escherichia coli and use of the monoclonal antibodies in a colony enzyme-linked immunosorbent assay. J. Clin. 11F11細(xì)胞Microbiol. 26: 2127-2131, 1988. PubMed: 3053764
