SK-LU-1細胞 
	
		是否是腫瘤細胞:	0
	
	
		物種來源:	人
	
	
		年限:	60 years
	
	
		細胞形態:	上皮樣
	
	
		生長狀態:	貼壁生長
	
	
		ATCC Number:	HTB-57?
	
	
		器官來源:	肺
	
	
		相關**:	腺癌
	
	
		運輸方式:	凍存運輸
	
	
		數量:	大量
	
	
		SK-LU-1細胞Designations:	SK-LU-1
	
	
		Depositors:	 G Trempe, LJ Old
	
	
		Biosafety Level:	1
	
	
		Shipped:	frozen
	
	
		Medium & Serum:	See Propagation
	
	
		Growth Properties:	adherent
	
	
		Organism:	Homo sapiens
	
	
		Morphology:	epithelial
	
	
		
	
	
		Source:	Organ: lung
	
	
		Disease: adenocarcinoma
	
	
		Permits/Forms:	In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
	
	
		Restrictions: SK-LU-1細胞The cells are distributed for research purposes only. The Memorial Sloan-Kettering Cancer Center releases the line subject to the following: 1.) The cells or their products must not be distributed to third parties. Commercial interests are the exclusive property of Memorial Sloan-Kettering Cancer Center. 2.) Any proposed commercial use of these cells must first be negotiated with The Director, Office of Industrial Affairs, Memorial Sloan-Kettering Cancer Center, 1275 York Avenue, New York, NY 10021; phone (212) 639-6181; FAX (212) 717-3439.
	
	
		Tumorigenic:	Yes
	
	
		Antigen Expression:	Blood Type O; Rh+; HLA Aw24, Aw32, B27, Bw41
	
	
		DNA Profile (STR):	Amelogenin: X
	
	
		CSF1PO: 10
	
	
		D13S317: 10
	
	
		D16S539: 8
	
	
		D5S818: 11
	
	
		D7S820: 9
	
	
		THO1: 7
	
	
		TPOX: 8,10
	
	
		vWA: 16,17
	
	
		Cytogenetic Analysis: SK-LU-1細胞The stemline chromosome number is hypotetraploid, with the 2S component occurring at 4.4%. Marker chromosomes 1p, t(1q;11q); 11q+; t(13;?); 16q+; t(12q; 18q); M10; t(2q;13q); i(15); and ?t(xp;21q) occurred in all S metaphases, and t(1p;?); t(1p;14q); t(16;?), and t(14;21) occurred in some. In addition, 4 to 9 small markers of unidentifiable origin occurred frequently. Chromosome No. 7 was generally hexasomic, X chromosomes were disomic, and normal No. 15 was absent. No Y chromosome was detected in the QM stained preparation.
	
	
		Isoenzymes:	AK-1, 1
	
	
		ES-D, 2
	
	
		G6PD, B
	
	
		GLO-I, 2
	
	
		Me-2, 1
	
	
		PGM1, 2
	
	
		PGM3, 1
	
	
		Age:	60 years
	
	
		Gender:	female
	
	
		Ethnicity:	Caucasian
	
	
		Comments:	Mycoplasma contamination was detected and eliminated in 1971.
	
	
		Propagation:	ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
	
	
		Temperature: 37.0°C
	
	
		Subculturing:	Protocol:
	
	
		Remove and discard culture medium.
	
	
		Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
	
	
		Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
	
	
		Note: SK-LU-1細胞To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37?C to facilitate dispersal.
	
	
		Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
	
	
		Add appropriate aliquots of the cell suspension to new culture vessels.
	
	
		Incubate cultures at 37?C.
	
	
		
	
	
		Subcultivation Ratio: A subcultivation ratio of 1:2 is recommended
	
	
		Medium Renewal: Twice per week
	
	
		Preservation:	Freeze medium: Complete growth medium, 95%; DMSO, 5%
	
	
		Storage temperature: liquid nitrogen vapor phase
	
	
		Related Products:	Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003
	
	
		recommended serum:ATCC 30-2020
	
	
		References:	22536: Fogh J, et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871
	
	
		23226: Pollack MS, et al. HLA-A, B, C and DR alloantigen expression on forty-six cultured human tumor cell lines. J. Natl. Cancer Inst. 66: 1003-1012, 1981. PubMed: 7017212