CCD-25Sk細胞
細胞類型： 成纖維細胞
運輸方式： 凍存運輸
是否是腫瘤細胞： 0
物種來源： 人
年限： 7 years
數(shù)量： 大量
細胞形態(tài)： 成纖維樣
器官來源： 皮膚
ATCC Number： CRL-1474?
相關**： 正常
生長狀態(tài)： 貼壁生長
CCD-25Sk細胞Designations: CCD-25Sk
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens
Morphology: fibroblast
Source: Organ: skin
Disease: normal
Cell Type: fibroblast
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
DNA Profile (STR): CCD-25Sk細胞Amelogenin: X,Y
CSF1PO: 10,12
D13S317: 10,14
D16S539: 9,11
D5S818: 12
D7S820: 8,11
THO1: 6,7
TPOX: 11,12
vWA: 17,18
Age: 7 years
Gender: male
Comments: The line was derived from normal skin of a patient that died from a high grade glioma.
This line is capable of at least 8 population doublings from the thawed ampule.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. CCD-25Sk細胞To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: 2 to 3 times per week
Remove medium, add fresh 0.25% trypsin solution, rinse, remove trypsin and let the culture sit at room temperature or at 37C until cells detach (about 10 minutes). Add fresh medium, aspirate and dispense into new flasks.
References: 32284: Loignon M, et al. A p53-independent pathway for induction of p21waf1cip1 and concomitant G1 arrest in UV-irradiated human skin fibroblasts. Cancer Res. 57: 3390-3394, 1997. CCD-25Sk細胞PubMed: 9270002
