P3X63Ag8U.1細胞
品系： BALB/c
數量： 大量
細胞形態： **樣
細胞類型： B**細胞
是否是腫瘤細胞： 0
物種來源： 小鼠
ATCC Number： CRL-1597?
相關**： 多發性骨髓瘤
運輸方式： 凍存運輸
生長狀態： 懸浮生長
Designations: P3X63Ag8U.1
P3X63Ag8U.1細胞Depositors: MD Scharff
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus
Morphology: lymphoblast
Source: Disease: plasmacytoma; myeloma
Strain: BALB/c
Cell Type: B lymphoblast;
Permits/Forms: P3X63Ag8U.1細胞In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Antigen Expression: H-2d
Gender: female
Comments: The cells do not synthesize gamma 1 chains.
They produce but do not secrete kappa chains.
The line was derived from the MOPC 21 - P3X63Ag8 lineage.
Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: P3X63Ag8U.1細胞The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Medium Renewal: 3 times per week
Cultures can be maintained by addition or replacement of fresh medium. Establish new cultures at 4 X 10 exp5 viable cells/ml and maintain below 1 X 10 exp6 cells/ml.
References: 1081: P3X63Ag8U.1細胞Yelton DE, et al. Fusion of mouse myeloma and spleen cells. Curr. Top. Microbiol. Immunol. 81: 1-7, 1978. PubMed: 567551
