L-132細胞 
	
		運輸方式:	凍存運輸
	
	
		器官來源:	其他
	
	
		ATCC Number:	CCL-5?
	
	
		數量:	大量
	
	
		生長狀態:	貼壁生長
	
	
		細胞形態:	上皮樣
	
	
		是否是腫瘤細胞:	0
	
	
		物種來源:	人
	
	
		##Caption##
	
	
		Designations:	L-132
	
	
		Depositors:	 EV Davis
	
	
		L-132細胞Biosafety Level:	2 [Cells contain Human papillomavirus ]
	
	
		Shipped:	frozen
	
	
		Medium & Serum:	See Propagation
	
	
		Growth Properties:	adherent
	
	
		Organism:	Homo sapiens
	
	
		Morphology:	epithelial
	
	
		
	
	
		Source:	Organ: HeLa contaminant
	
	
		Cellular Products:	keratin
	
	
		Permits/Forms:	In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
	
	
		DNA Profile (STR):	Amelogenin: X
	
	
		CSF1PO: 9,10
	
	
		D13S317: 12,13.3
	
	
		D16S539: 9,10
	
	
		D5S818: 11,12
	
	
		D7S820: 8,12
	
	
		L-132細胞THO1: 7
	
	
		TPOX: 8,12
	
	
		vWA: 16,18
	
	
		Isoenzymes:	G6PD, A
	
	
		HeLa Markers:	Y
	
	
		Comments:	This line was originally thought to be derived from embryonic lung tissue, but was subsequently found, based on isoenzyme analysis, HeLa marker chromosomes, and DNA fingerprinting, to have been established via HeLa cell contamination.
	
	
		The cells are positive for keratin by immunoperoxidase staining.
	
	
		Propagation:	ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
	
	
		Temperature: 37.0°C
	
	
		Subculturing:	Subcultivation Ratio:L-132細胞 A subcultivation ratio of 1:2 to 1:8 is recommended
	
	
		Medium Renewal: Twice per week
	
	
		Remove medium, add fresh 0.25% trypsin solution for 1 to 3 minutes. Remove trypsin and let culture sit at room temperature for 10 to 15 minutes. Add fresh medium, aspirate and dispense into new flasks.
	
	
		References:	18385: Didier ES, et al. Characterization of Encephalitozoon (Septata) intestinailis isolates cultured from nasal mucosa and bronchoalveolar lavage fluids of two AIDS patients. J. Eukaryot. Microbiol. 43: 34-43, 1996. PubMed: 8563708
	
	
		22127: Erfle V, Mellert W. Human cell line LC5 and its use. US Patent 5,582,967 dated Dec 10 1996
	
	
		22222: . . Fed. Proc. 19: 386, 1960.
	
	
		26042: . . Science 133: 2059, 1961.
	
	
		32706: L-132細胞Tresnan DB, et al. Feline aminopeptidase N serves as a receptor for feline, canine, porcine, and human coronaviruses in serogroup I. J. Virol. 70: 8669-8674, 1996. PubMed: 8970993