Rat2細胞
細胞形態： 成纖維樣
是否是腫瘤細胞： 0
物種來源： 大鼠
數量： 大量
生長狀態： 貼壁生長
品系： Fischer
年限： embryo; fetus
ATCC Number： CRL-1764?
相關**： 正常
運輸方式： 凍存運輸
Designations: Rat2
Depositors: B Ahrens
Rat2細胞Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Rattus norvegicus deposited as Rattus sp.
Morphology: fibroblast
Source: Disease: normal
Strain: Fischer
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Age: embryo; fetus
Comments: This line was derived from the a 5-bromo-2'-deoxyuridine resistant strain of the Fischer rat fibroblast 3T3 like cell line, Rat1 (developed by W.C. Topp).
Rat2 lacks detectable nuclear thymidine kinase, is highly transfectable by exogenous DNA and is phenotypically normal.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. Rat2細胞To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: Twice per week
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Preservation: Culture medium, 95%; DMSO, 5%
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2020
References: 1835: Rat2細胞Topp WC. Normal rat cell lines deficient in nuclear thymidine kinase. Virology 113: 408-411, 1981. PubMed: 7269249
33037: Peng M, et al. Partial inhibition of Na+/K+-ATPase by ouabain induces the Ca2+-dependent expressions of early-response genes in cardiac myocytes. J. Biol. Chem. 271: 10372-10378, 1996. PubMed: 8626609
