JB6 Cl 41-5a細(xì)胞

生長(zhǎng)狀態(tài)： 貼壁生長(zhǎng)

細(xì)胞形態(tài)： 上皮樣

數(shù)量： 大量

ATCC Number： CRL-2010?

相關(guān)**： 正常

是否是腫瘤細(xì)胞： 0

物種來(lái)源： 小鼠

運(yùn)輸方式： 凍存運(yùn)輸

器官來(lái)源： 皮膚

年限： newborn

品系： BALB/c

組織來(lái)源： epidermis

Designations: JB6 Cl 41-5a

JB6 Cl 41-5a細(xì)胞Depositors: NH Colburn

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Mus musculus

Morphology: epithelial

Source: Strain: BALB/c

Organ: skin

Tissue: epidermis

Disease: normal

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. JB6 Cl 41-5a細(xì)胞Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Tumorigenic: No

Age: newborn

Comments: The line is sensitive to promotion of transformation (P+) by phorbol esters and other tumor promoters; this is one of several lines that are useful in studies of the molecular events in tumor promotion and for development of promotion assays (see also ATCC CRL-2007 - JB6 Cl 30-7b, ATCC CRL-2002 - RT101 and ATCC CRL-2012 - T36274).

The JB6 Cl 41-5a cell line was established from primary cultures of neonatal BALB/c epidermal cells that had been treated with dimethylsulfoxide (DMSO, 0.01 to 0.1 %).

The cells must be carried at low density to avoid spontaneous transformation.

The cells should be subcultured for at least two passages prior to testing in agar for tumor promotor induced transformation.

For the Anchorage Independent Transformation Assay, suspend 10000 cells in 0.33% agar medium containing 8% fetal bovine serum, with or without added tumor promoter (e.g., 10 ng/ml TPA).

Incubate at 37C, and score colonies greater than 8 cells at 14 days.

JB6 Cl 41-5a細(xì)胞Propagation: ATCC complete growth medium: Minimum essential medium (Eagle) with 2 mM L-glutamine and Earle's BSS adjusted to contain 1.5 g/L sodium bicarbonate, 0.1 mM non-essential amino acids, and 1.0 mM sodium pyruvate, 95%; heat-inactivated fetal bovine serum, 5%

Temperature: 37.0°C

Subculturing: Protocol: Remove medium, rinse with fresh 0.25% trypsin, 0.53 mM EDTA solution, remove the trypsin solution and let the culture sit at room temperature (or at 37C) until the cells detach (about 5 TO 15 minutes). Add fresh medium, aspirate and dispense into new flasks. Do not allow the cells to become confluent.

Subcultivation Ratio: An inoculation density of 2 X 10 exp4 viable cells per 25 sq. cm. flask is recommended

Medium Renewal: 2 to 3 times per week

Preservation: Culture medium with an additional 5% fetal bovine serum, 95%; DMSO, 5%

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003

References: 22309: Colburn NH, et al. A cell culture assay for tumor-promoter-dependent progression toward neoplastic phenotype: detection of tumor promoters and promotion inhibitors. Teratog. Carcinog. Mutagen. 1: 87-96, 1980. PubMed: 6119803

22350: Sun Y, et al. Dosage-dependent dominance over wild-type p53 of a mutant p53 isolated from nasopharyngeal carcinoma. FASEB J. 7: 944-950, 1993. PubMed: 8344492

22427: Colburn NH, et al. JB6 Cl 41-5a細(xì)胞Correlation of anchorage-independent growth with tumorigenicity of chemically transformed mouse epidermal cells. Cancer Res. 38: 624-634, 1978. PubMed: 626967

22599: Dong Z, et al. Blocking of tumor promoter-induced AP-1 activity inhibits induced transformation in JB6 mouse epidermal cells. Proc. Natl. Acad. Sci. USA 91: 609-613, 1984. PubMed: 8290571

22908: Colburn NH, et al. Tumour promoter induces anchorage independence irreversibly. Nature 281: 589-591, 1979. PubMed: 492322

22959: Bernstein LR, Colburn NH. AP1/jun function is differentially induced in promotion-sensitive and resistant JB6 cells. Science 244: 566-569, 1989. PubMed: 2541502

23279: Colburn NH, et al. Dissociation of mitogenesis and late-stage promotion of tumor cell phenotype by phorbol esters: mitogen-resistant variants are sensitive to promotion. Proc. Natl. Acad. Sci. USA 78: 6912-6916, 1981. PubMed: 6947266

23336: Sun Y, et al. Progression toward tumor cell phenotype is enhanced by overexpression of a mutant p53 tumor-suppressor gene isolated from nasopharyngeal carcinoma. Proc. Natl. Acad. Sci. USA 90: 2827-2831, 1993. PubMed: 8464896