CVC.1細胞
運輸方式： 凍存運輸
ATCC Number： TIB-135?
數(shù)量： 大量
是否是腫瘤細胞： 0
物種來源： 小鼠
生長狀態(tài)： 懸浮生長
細胞形態(tài)： **樣
**類型： IgG1
Designations: CVC.1
Depositors: FM Brodsky, P Parham
CVC.1細胞Isotype: IgG1
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products: immunoglobulin; monoclonal antibody; against bovine brain clathrin (light chain, 36000 dalton)
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Comments: Animals were immunized with bovine brain clathrin.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
CVC.1細胞Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC Hybri-Care Medium, Catalog No. 46-X. Hybri-Care Medium is supplied as a powder and should be reconstituted in 1 L cell-culture-grade water. To make the complete growth medium, add the following components to the base medium:
fetal bovine serum to a final concentration of 10%
1.5 g/L sodium bicarbonate for use with 5% CO2 in air atmosphere.
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 x 10 exp5 cells/ml and maintain between 1 x 10 exp5 and 1 x 10 exp6 cells/ml.
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 46-X
References: 966: Kirchhausen T, et al. CVC.1細胞Location and distribution of the light chains in clathrin trimers. Proc. Natl. Acad. Sci. USA 80: 2481-2485, 1983. PubMed: 6189124
974: Brodsky FM, Parham P. Polymorphism in clathrin light chains from different tissues. J. Mol. Biol. 167: 197-204, 1983. PubMed: 6864801
