tsc2 ang1細胞
ATCC Number： CRL-2620?
數量： 大量
相關**： 其他**
生長狀態： 貼壁生長
是否是腫瘤細胞： 0
物種來源： 小鼠
運輸方式： 凍存運輸
Designations: tsc2 ang1
Depositors: JL Arbiser
Biosafety Level: 1
tsc2 ang1細胞Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Mus musculus deposited as mouse
Morphology:
Source: Disease: cutaneous sarcoma
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Tumorigenic: Yes
Comments: tsc2 ang1細胞Tuberous sclerosis (TSC) is an autosomal dominant genetic disorder caused by mutations in either TSC1 or TSC2. Mice heterozygous for Tsc2 were developed as a murine model of Tsc2 disease using a gene targeting approach. [53493]
The tsc2 ang1 cell line was derived in 1998 from a cutaneous sarcoma taken from a Tsc2+/- mouse heterozygous for tuberin.
The cell line is a useful model of TSC. It is the only genetically defined tuberous sclerosis cell line available that forms tumors. It can be used for drug testing and for angiogenesis studies.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:5 is recommended
Medium Renewal: tsc2 ang1細胞Every 2 to 3 days
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Preservation: culture medium 95%; DMSO, 5%
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2020
References: 53493: Onda H, et al. Tsc2(+/-) mice develop tumors in multiple sites that express gelsolin and are influenced by genetic background. J. Clin. Invest. 104: 687-695, 1999.tsc2 ang1細胞 PubMed: 10491404
