PFHR 9細(xì)胞
運(yùn)輸方式： 凍存運(yùn)輸
數(shù)量： 大量
ATCC Number： CRL-2423?
相關(guān)**： 其他**
生長狀態(tài)： 貼壁生長
細(xì)胞形態(tài)： 上皮樣
是否是腫瘤細(xì)胞： 0
物種來源： 小鼠
年限： embryo
Designations: PFHR 9 [PFHR-9]
Depositors: A Chung
Biosafety Level: 1
PFHR 9細(xì)胞Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Mus musculus deposited as mouse
Morphology: epithelial
Source: Disease: embryonal carcinoma
Cellular Products: laminin-1; entactin-1; perlecan and collagen IV
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Tumorigenic: No
Age: embryo
Comments: PFHR 9細(xì)胞PFHR 9 (PFHR-9)is a differentiated cell line isolated as a HAT-resistant colony of the undifferentiated mouse embryonal carcinoma PCC4F in 1974.
While PCC4F cells grow in suspension, the PRHR cell line is adherent. The cells are epithelial-like and have numerous vacuoles. PFHR 9 cells have a reported doubling time of 12 hours.
The PFHR 9 cells were not tumorigenic when injected subcutaneously into 129/Sv mice whereas the parental line did produce tumors.
The cells produce basement membrane proteins - laminin-1; entactin-1; perlecan and collagen IV.
The cell line is useful for the production of basement membrane proteins and as a model to study the assembly and secretion of these proteins.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended
Medium Renewal: Every 2 to 3 days
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution.PFHR 9細(xì)胞 Allow the flask to sit at room temperature (or at 37C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Preservation: culture medium 95%; DMSO, 5%
Doubling Time: 12 hrs
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2020
References: 38748: Chung AE, et al. Morphological and biochemical observations on cells derived from the in vitro differentiation of the embryonal carcinoma cell line PCC4-F. Cancer Res. 37: 2072-2081, 1977. PFHR 9細(xì)胞PubMed: 558822
