D10.G4.1細胞
運輸方式： 凍存運輸
細胞類型： 其他細胞類型
是否是腫瘤細胞： 0
物種來源： 小鼠
年限： *****
器官來源： **結
數量： 大量
細胞形態： **樣
生長狀態： 懸浮生長
ATCC Number： TIB-224?
品系： AKR/J
Designations: D10.G4.1
D10.G4.1細胞Depositors: C Janeway, J Kaye, P Conrad
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus
Morphology: lymphoblast
Source: Organ: lymph node
Strain: AKR/J
Cell Type: helper T lymphocyte (Th-2);
Cellular Products: interleukin 4 (interleukin-4, IL-4); interleukin 5 (interleukin-5, IL-5)
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Receptors: interleukin 1 (IL-1), expressed
interleukin 1 (IL-1)
Antigen Expression: H-2k
Age: *****
D10.G4.1細胞Gender: male
Comments: The cells are specific for egg white conalbumin (Sigma No. C0755) and alloreactive to I-A b, v.
They can be used to assay IL-1 because they proliferate minimally in response to Con A in the absence of IL-1 or feeder cells.
Supernatant media from Con A stimulated D10.G4.1 cells contain IL-4 and IL-5.
Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium:
10% T-STIM with Con A (rat IL-2 culture supplement ) Becton Dickinson, Catalog No. 354115)
10% fetal bovine serum
0.05 mM 2-mercaptoethanol
10 pg/ml mouse IL-1 alpha (R&D Systems, Catalog No. 400ML)
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Subculturing: Protocol: Subculture actively growing suspension cultures before they have reached 1 X 10(6) cells/ml or the IL-1 and IL-2 will be rapidly depleted and the cells will lose viability. Use inoculation densities of 2 X 10 (5) viable cells/ml.
Medium Renewal: Twice per week
Preservation: Freeze medium: D10.G4.1細胞Complete growth medium supplemented with an additional 10% FBS and 5% (v/v) DMSO.
Storage temperature: liquid nitrogen vapor phase
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
recommended serum:ATCC 30-2020
References: 1763: Kupper T, et al. Growth of an interleukin-2/interleukin 4-dependent T cell line induced by granulocyte-macrophage colony-stimulating factor (GM-CSF). J. Immunol. 138: 4288-4292, 1987. PubMed: 2953804
22166: . . Immunol. Rev. 81: 40, 1984.
22205: Kaye J, Janeway CA J. Induction of receptors for interleukin 2 requires T cell Ag:Ia receptor crosslinking and interleukin 1. Lymphokine Res. 3: 175-182, 1984. PubMed: 6436584
22359: Watson J. Continuous proliferation of murine antigen-specific helper T lymphocytes in culture. J. Exp. Med. 150: 1510-1519, 1979. PubMed: 92524
22817: Kaye J, et al. Both a monoclonal antibody and antisera specific for determinants unique to individual cloned helper T cell lines can substitute for antigen and antigen-presenting cells in the activation of T cells. J. Exp. Med. 158: 836-856, 1983. PubMed: 6193236
23435: Kaye J, Janeway CA Jr.. The Fab fragment of a directly activating monoclonal antibody that precipitates a disulfide-linked heterodimer from a helper T cell clone blocks activation by either allogeneic Ia or antigen and self-Ia. J. Exp. Med. 159: 1397-1412, 1984. PubMed: 6232337
23451: Kaye J, et al. Growth of a cloned helper T cell line induced by a monoclonal antibody specific for the antigen receptor: interleukin 1 is required for the expression of receptors for interleukin 2. J. Immunol. 133: 1339-1345, 1984. PubMed: 6235287
23452: Kaye J, Janeway CA Jr.. D10.G4.1細胞The alpha- and beta-subunits of a murine T cell antigen/Ia receptor have a molecular weight of 31,000 in the absence of N-linked glycosylation. J. Immunol. 133: 2291-2300, 1984. PubMed: 6237148
23470: Swain SL, et al. Role of antigen in the B cell response. Specific antigen and the lymphokine IL-5 synergize to drive B cell lymphoma proliferation and differentiation to Ig secretion. J. Immunol. 140: 4224-4230, 1988. PubMed: 2453572
