3C7.2細(xì)胞

是否是腫瘤細(xì)胞： 0

物種來源： 小鼠

運(yùn)輸方式： 凍存運(yùn)輸

ATCC Number： CRL-1959?

生長狀態(tài)： 懸浮生長

數(shù)量： 大量

細(xì)胞形態(tài)： **樣

**類型： IgG1; kappa light chain

Designations: 3C7.2

Depositors: MF Heyworth

Isotype: IgG1; kappa light chain

3C7.2細(xì)胞Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)

Morphology: lymphoblast

Source: Cell Type: hybridoma: B lymphocyte;

Cellular Products: immunoglobulin; monoclonal antibody; against Giardia muris trophozoites

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Comments: 3C7.2細(xì)胞Animals were immunized with G. muris trophozoites.

Spleen cells were fused with P3/NSI/1-Ag4-1 myeloma cells.

The antibody reacts with G. muris trophozoite surfaces.

Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 80%;NCTC 109 medium, 10%;(Add 1 mM oxaloacetic acid, 0.45 mM sodium pyruvate, 4 mM L-glutamine, 0.1 mM hypoxanthine, 0.01 mM thymidine and 10 units/ml bovine insulin to this mixture);horse serum, 10%

Subculturing: Medium Renewal: Every 2 to 3 days

Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.

References: 22728: 3C7.2細(xì)胞Heyworth MF, et al. Generation and characterization of monoclonal antibodies against Giardia muris trophozoites. Immunology 68: 341-345, 1989. PubMed: 2592009