4C9細胞
運輸方式： 凍存運輸
是否是腫瘤細胞： 0
物種來源： 小鼠
ATCC Number： CRL-2437?
生長狀態： 懸浮生長
細胞形態： **樣
**類型： IgG1
數量： 大量
Designations: 4C9
Depositors: PJ Bjorkman
Isotype: IgG1
Biosafety Level: 1
4C9細胞Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products: immunoglobulin; monoclonal antibody; against rat beta2 microglobulin
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Comments: 4C9細胞The cell line was established by Louis Gastinel and Pamela Bjorkman in 1992.
Animals were immunized with and twice boosted with soluble neonatal Fc receptor (FcRn) in an adjuvant. Three days before fusion one mouse was boosted with purified FcRn.
Spleen cells were fused with HL-1 Friendly myeloma cells (a derivative of P3X63Ag8.653 that is deficient in both hypoxanthine phosphoribosyl transferase [HPRT] and adenine phosphoribosyl transferase [APRT]).
The antibody recognizes rat beta 2-microglobulin alone or complexed with FcRn heavy chains.
FcRn is a heterodimer composed of a membrane bound heavy chain attached noncovalently to beta 2-microgloublin. It is structurally similar to class I major histocompatibility (MHC) molecules.
This antibody is used in studies of functions of the MHC class I light chain.
Propagation: ATCC complete growth medium: HL-1 medium supplemented with 4 mM L-glutamine, 1 mM sodium pyruvate and 1% fetal bovine serum. HL-1 medium can be obtained from from Lonza (catalog number 77201).
Temperature: 37.0°C
Subculturing: 4C9細胞Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 X 10 exp5 viable cells/ml.
Maintain cell density between 1 X 10 exp5 and 1 X 10 exp6 viable cells/ml.
Preservation: HL-1 Complete Serum-free Medium, 75%; fetal bovine serum, 20%; DMSO, 5%.
Related Products: recommended serum:ATCC 30-2020
References: 39787: Raghavan M, et al. Investigation of the interaction between the class I MHC-related Fc receptor and its immunoglobulin G ligand. Immunity 1: 303-315, 1994. 4C9細胞PubMed: 7889418
