BJ細胞
器官來源： 皮膚
運輸方式： 凍存運輸
年限： newborn
細胞形態： 成纖維樣
生長狀態： 貼壁生長
數量： 大量
ATCC Number： CRL-2522?
相關**： 正常
細胞類型： 成纖維細胞
是否是腫瘤細胞： 0
物種來源： 人
BJ細胞Designations: BJ
Depositors: JR Smith
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens deposited as human
Morphology: fibroblast
Source: Organ: skin; foreskin
Disease: normal
Cell Type: fibroblast
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Applications: transfection host
DNA Profile (STR): Amelogenin: X,Y
CSF1PO: 10,12
D13S317: 8,9
D16S539: 9,13
D5S818: 12
D7S820: 11,12
THO1: 7,8
TPOX: 10,11
BJ細胞vWA: 16,18
Cytogenetic Analysis: [49835]
Age: newborn
Gender: male
Comments: The line was established from skin taken from normal foreskin.
The BJ cell line has a long lifespan in comparison with other normal human fibroblast cell lines.
A frozen ampule at population doubling 2.3 was received at the ATCC in April, 2000.
These cells have a reported normal diploid karyotype at population doubling 61 but an abnormal karyotype at population doubling 82. [49835]
They are telomerase negative. [44175]
The cells may be used for stable transfection studies. [47354]
Cells from ATCC ampules have the capacity to proliferate to a maximum of 72 population doublings before the onset of senescence.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Subculturing: Protocol:
Remove and discard culture medium.
BJ細胞Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37?C to facilitate dispersal.
Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37?C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:9 is recommended
Medium Renewal: Every 2 to 3 days
Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003
recommended serum:ATCC 30-2020
References: 44175: Yi X, et al. Both transcriptional and posttranscriptional mechanisms regulate human telomerase template RNA levels. Mol. Cell. Biol. 19(6): 3989-3997, 1999. BJ細胞PubMed: 10330139
47354: Bodnar AG, et al. Extension of life-span by introduction of telomerase into normal human cells. Science 279: 349-352, 1998. PubMed: 9454332
48461: Huffman KE, et al. Telomere shortening is proportional to the size of the G-rich telomeric 3'-overhang. J. Biol. Chem. 275: 19719-19722, 2000. PubMed: 10787419
49835: Morales CP, et al. Absence of cancer-associated changes in human fibroblasts immortalized with telomerase. Nat. Genet. 21: 115-118, 1999. PubMed: 9916803
