CL18/6細(xì)胞
生長狀態(tài)： 懸浮生長
細(xì)胞形態(tài)： **樣
**類型： IgG1
ATCC Number： CRL-2518?
是否是腫瘤細(xì)胞： 0
物種來源： 小鼠
運輸方式： 凍存運輸
數(shù)量： 大量
Designations: CL18/6
Depositors: C Smith
Isotype: IgG1
Biosafety Level: 1
CL18/6細(xì)胞Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products: immunoglobulin; monoclonal antibody; against canine intracellular adhesion molecule 1 (ICAM-1).
Permits/Forms: CL18/6細(xì)胞In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments: Animals were immunized with canine endothelial cells that had been stimulated with lipopolysaccharide (LPS).
Spleen cells were fused with P3X63Ag8.653 mouse myeloma cells.
The antibody reacts with canine intercellular adhesion molecule 1 (ICAM-1).
Antibody produced by CL18/6 (ATCC CRL-2518), but not CL18/1 (ATCC CRL-2517), blocks adherence of canine neutrophils to lipopolysaccharide (LPS) stimulated canine jugular vein endothelial cells. [47314]
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
CL18/6細(xì)胞Temperature: 37.0°C
Subculturing: Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 X 10 exp5 viable cells/ml.
Maintain cell density between 5 X 10 exp4 and 1 X 10 exp6 viable cells/ml.
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
recommended serum:ATCC 30-2020
References: 47314: Smith CW, et al. Adherence of neutrophils to canine cardiac myocytes in vitro is dependent on intercellular adhesion molecule-1. J. Clin. Invest. 88: 1216-1223, 1991. CL18/6細(xì)胞PubMed: 1680878
