ICR 134 (RPH67.134c2) [Diploid Frog]細(xì)胞
數(shù)量： 大量
年限： embryo
ATCC Number： CCL-128?
器官來源： 其他
運輸方式： 凍存運輸
細(xì)胞形態(tài)： 上皮樣
生長狀態(tài)： 貼壁生長
是否是腫瘤細(xì)胞： 0
物種來源： 其他
ICR 134 (RPH67.134c2) [Diploid Frog]細(xì)胞Designations: ICR 134 (RPH67.134c2) [Diploid Frog]
Depositors: J Freed, L Mezger-Freed
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Rana pipiens
Morphology: epithelial
Source: Organ: gynogenetic haploid embryo
Permits/Forms: ICR 134 (RPH67.134c2) [Diploid Frog]細(xì)胞In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Virus Resistance: poliovirus; vesicular stomatitis (Indiana); herpes simplex; vaccinia
Cytogenetic Analysis: near diploid
Age: embryo
Comments: This line was derived from the minced tissues of stage 17 gynogenetic haploid embryos of the Vermont population of Rana pipiens.
The line was cloned at the 11th passage and the cloned line (RPH67.134c2) proved to be near diploid.
For haploid cells, see ICR 2A (ATCC CCL-145).
Propagation: ICR 134 (RPH67.134c2) [Diploid Frog]細(xì)胞ATCC complete growth medium: Leibovitz's L-15 medium, 50%; distilled water, 40%; fetal bovine serum, 10%. Incubate at 25C in air without CO2.
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: 24 hours after thawing; from then on at time of subculture
Remove medium, add fresh 0.25% trypsin for 1 to 3 minutes, remove the trypsin and let the culture stand for 5 to 10 minutes at room temperature. Add fresh culture medium, aspirate and dispense into new flasks.
References: 21860: . Biology of amphibian tumors. New York: Springer-Verlag; 1969.
22249: . . Methods Cell Physiol. 4: 20-46, 1970.
26296: . ICR 134 (RPH67.134c2) [Diploid Frog]細(xì)胞Biology of amphibian tumors. New York: Springer-Verlag; 1969.
