C1R-neo細胞

生長狀態： 懸浮生長

細胞類型： B**細胞

是否是腫瘤細胞： 0

物種來源： 人

數量： 大量

ATCC Number： CRL-2369?

運輸方式： 凍存運輸

器官來源： 外周血

細胞形態： **樣

Designations: C1R-neo

Depositors: F Grumet

C1R-neo細胞Biosafety Level: 2 [Cells contain herpesvirus ]

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Homo sapiens

Morphology: lymphoblast

Source: Organ: peripheral blood

Cell Type: B lymphoblast; Epstein-Barr virus (EBV) transformed

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DNA Profile (STR): Amelogenin: X

CSF1PO: 6,10

D13S317: 11,13

D16S539: 9,13

C1R-neo細胞D5S818: 10,13

D7S820: 7,12

THO1: 8

TPOX: 8

vWA: 17

Comments: C1R-neo is a stable transfectant cell line established in 1987 by transfection by electroporation of the C1R cell line with a modified neomycin drug-resistant eukaryotic vector, pSP65-Neo. The vector did not carry an insert. [38581]

C1R-neo does not secrete or express HLA B7.

C1R is a human B-cell lymphoblastoid line lacking surface HLA A and B antigens. C1R was derived from Hmy.2 B-LCL by gamma irradiation followed by selection for Class I monoclonal antibodies and complement. [23312]

C1R-neo may be used as a control for C1R-sB7 (CRL-2370), in CTL experiments and for producing control supernatants.

Propagation:C1R-neo細胞 ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Iscove's Modified Dulbecco's Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Temperature: 37.0°C

Subculturing: Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)

Cultures can be maintained by addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension in fresh medium at 5 X 10(5) viable cells/ml.

Maintain cultures at cell concentrations between 5 X 10(5) and 2 X 10(6) viable cells/ml.

Preservation: culture medium 95%; DMSO, 5%

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2005

recommended serum:ATCC 30-2020

parental cell line:ATCC CRL-1993

derived from same cell line:C1R-neo細胞ATCC CRL-2370

derived from same cell line:ATCC CRL-2371

References: 23312: Storkus WJ, et al. Reversal of natural killing susceptibility in target cells expressing transfected class I HLA genes. Proc. Natl. Acad. Sci. USA 86: 2361-2364, 1989. PubMed: 2784569

38581: Grumet FC, et al. Soluble form of an HLA-B7 class I antigen specifically suppresses humoral alloimmunization. Hum. Immunol. 40: 228-234, 1994. PubMed: 7960967

38582: Hiraki DD, et al. Bioengineered soluble HLA-B7. Genesis, characterization, and occurrence of dimerization. Hum. Immunol. 40: 235-246, 1994. PubMed: 7960968