GH4C1細胞
年限： 7 months
運輸方式： 凍存運輸
器官來源： 垂體
生長狀態： 漂浮生長
是否是腫瘤細胞： 0
物種來源： 大鼠
品系： Wistar-Furth
細胞形態： 上皮樣
ATCC Number： CCL-82.2?
數量： 大量
相關**： 腫瘤
Designations: GH4C1
GH4C1細胞Depositors: AH Tashjian
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: loosely adherent with floating clusters
Organism: Rattus norvegicus deposited as Rattus sp.
Morphology: epithelial
Source: Organ: pituitary
Strain: Wistar-Furth
Disease: tumor
Cellular Products: prolactin; growth hormone (somatotrophin)
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Cytogenetic Analysis: modal number = 65; range = 60 to 68.
This is a hypertriploid rat cell line with the modal number of 65, occurring in 32% of cells. However, cells with 67 chromosomes also occurred at a high rate (22%). GH4C1細胞Cells with a higher ploidy count occurred at 0.6%. There were 25 to 30 marker chromosomes per cell including paired T(1;2-), single 4p+, chromosome 2 with the deleted q16-q24 (?), 11p+ and about 15 other constitutive markers. Only a single copy of the X chromosome was identified. Other characteristics included consistent single copies for chromosomes 1 and 3, double copies for chromosomes 5 and 8, and more than four copies for chromosome 7.
Age: 7 months
Gender: female
Comments: The GH4C1 cell line was developed in 1972 by A.H. Tashjian, Jr. from serially propagated GH3 (ATCC CCL-82.1) cells that suddenly produced little or no detectable levels of growth hormone.
GH4C1 cells differed from GH3 cells in that basal levels of growth hormone production were low (0.06 or less hormone/mg cell protein/24 hours.
Prolactin is also produced at a rate of 0.006 to 0.012 mg hormone/mg cell protein/24 hours.
Thyrotropin-releasing Hormone (TRH) stimulates prolactin synthesis and secretion.
Cloned cDNA from GH4C1 cells has been used to investigate the structure and regulation of the rat TRH receptor.
This line provides a system for studying the molecular mechanisms of action of hypothalamic peptides on growth hormone and prolactin release and synthesis.
The cells provide a system for studying the molecular mechanisms of receptor modulation and signal transduction in pituitary - derived cells.
GH4C1細胞They may be used for electrophysiological studies on plasma membrane calcium channels and also for studies of intracellular calcium homeostasis in secretory cells.
These cells have a plating efficiency of approximately 22% in the recommended culture medium.
Propagation: ATCC complete growth medium: Ham's F10 medium, 82.5%; horse serum, 15%; fetal bovine serum, 2.5%
Temperature: 37.0°C
Subculturing: Protocol:
Remove and discard culture medium.. Sometimes many cells are floating, they can be harvested by centrifugation of medium instead of discarding it.
Add 2.0 to 3.0 ml of 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37?C.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:20 is recommended
Medium Renewal: 2 to 3 times per week
Preservation: Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Related Products: recommended serum:ATCC 30-2020
recommended serum:ATCC 30-2040
parental cell line:ATCC CCL-82.1
References: 881: Tashjian AH Jr., et al. GH4C1細胞Establishment of clonal strains of rat pituitary tumor cells that secrete growth hormone. Endocrinology 82: 342-352, 1968. PubMed: 4951281
21885: . Tissue Culture: methods and applications. New York: Academic Press; 1973.
22421: Takemoto H, et al. Adrenotropic activity of mammo-somatotropic tumors in rats and mice. I. Biologic aspects. Cancer Res. 22: 917-924, 1962. PubMed: 13984648
22573: Tashjian AH Jr.. Clonal strains of hormone-producing pituitary cells. Methods Enzymol. 58: 527-535, 1979. PubMed: 218079
23514: Zhao D, et al. Molecular cloning of a complementary deoxyribonucleic acid encoding the thyrotropin-releasing hormone receptor and regulation of its messenger ribonucleic acid in rat GH cells [published erratum appears in Endocrinology 132: 2658, 1993]. Endocrinology 130: 3529-3536, 1992. PubMed: 1317787
23543: Albert PR, Tashjian AH Jr.. Relationship of thyrotropin-releasing hormone-induced spike and plateau phases in cytosolic free Ca2+ concentrations to hormone secretion. Selective blockade using ionomycin and nifedipine. J. Biol. Chem. 259: 15350-15363, 1984. PubMed: 6439720
