VIII-6G10細胞
細胞形態： **樣
**類型： IgG1; kappa light chain
ATCC Number： HB-10519?
是否是腫瘤細胞： 0
物種來源： 小鼠
生長狀態： 懸浮生長
數量： 大量
運輸方式： 凍存運輸
Designations: VIII-6G10
Depositors: Fred Hutchinson Cancer Res. Cntr.
VIII-6G10細胞Isotype: IgG1; kappa light chain
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products: immunoglobulin; monoclonal antibody; against vascular cell adhesion molecule 1 (VCAM-1, VCAM 1) of human and macaque
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VIII-6G10細胞Tumorigenic: Yes
Comments: Animals were immunized with IL-4 and IL-1 beta activated endothelial cells isolated from mesenteric lymph nodes of Macaca nemestrina.
Spleen cells were fused with NS-1 myeloma cells.
The antibody is specific for VCAM-1 and blocks up to 90% of lymphocyte adhesion to microvascular endothelial cells.
Propagation: ATCC complete growth medium: RPMI 1640 medium with 25 mM HEPES, 2 mM L-glutamine and 1 mM sodium pyruvate, 90%; fetal bovine serum, 10%
Temperature: 37.0°C
Subculturing: Medium Renewal: VIII-6G10細胞Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Preservation: Culture medium, 95%; DMSO, 5%
References: 22026: Masinovsky B, et al. IL-4 and TNF induce mAb 6G10-recognized expression on bone marrow stromal cells. US Patent 5,206,345 dated Apr 27 1993
23475: Masinovsky B, et al. IL-4 acts synergistically with IL-1 beta to promote lymphocyte adhesion to microvascular endothelium by induction of vascular cell adhesion molecule-1. J. Immunol. 145: 2886-2895, 1990. VIII-6G10細胞PubMed: 1698865
