4B2細(xì)胞
運(yùn)輸方式： 凍存運(yùn)輸
數(shù)量： 大量
ATCC Number： HB-196?
是否是腫瘤細(xì)胞： 0
物種來源： 小鼠
生長狀態(tài)： 懸浮生長
細(xì)胞形態(tài)： **樣
**類型： IgG2a
Designations: 4B2
Depositors: RM Steinman
Isotype: IgG2a
Biosafety Level: 1
4B2細(xì)胞Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products: 4B2細(xì)胞immunoglobulin; monoclonal antibody; against the human T200 common leukocyte antigen (CD45)
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Tumorigenic: Yes
Comments: Animals were immunized with human T cells.
Spleen cells were fused with P3U1 myeloma cells.
The antibody reacts with all types of human leukocytes, but not with non-leukocytes.
The antibody binds to protein A.
Tested and found negative for ectromelia virus (mousepox).
Propagation: 4B2細(xì)胞ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
References: 21881: . Leucocyte typing III: white cell differentiation antigens. Oxford: Oxford Univ.4B2細(xì)胞 Press; 1987.
