FW11-9-2細(xì)胞

細(xì)胞形態(tài)： **樣

**類型： IgG2a

運(yùn)輸方式： 凍存運(yùn)輸

數(shù)量： 大量

生長狀態(tài)： 懸浮生長

ATCC Number： HB-256?

是否是腫瘤細(xì)胞： 0

物種來源： 小鼠

Designations: FW11-9-2

Depositors: CR Mackay

Isotype: IgG2a

FW11-9-2細(xì)胞Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)

FW11-9-2細(xì)胞Morphology: lymphoblast

Source: Cell Type: hybridoma: B lymphocyte;

Cellular Products: immunoglobulin; monoclonal antibody; against the v6 variant of human CD44

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Comments: FW11-9-2細(xì)胞Animals were immunized with a fusion protein corresponding to the variant region of human CD44 (exons 3v to 10v).

Spleen cells were fused with Sp2/0-Ag14 myeloma cells.

The antibody is specific for the product of the 6v exon.

Propagation: ATCC complete growth medium: Iscove's modified Dulbecco's medium with 2 mM L-glutamine, 90%; fetal bovine serum, 10%

Subculturing: Medium Renewal: Every 2 to 3 days

Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.

Preservation: Culture medium, 95%; DMSO, 5%

References: 22355: Mackay CR, et al. FW11-9-2細(xì)胞Expression and modulation of CD44 variant isoforms in humans. J. Cell Biol. 124: 71-82, 1994. PubMed: 7507492