FBHE細(xì)胞
細(xì)胞類型： 內(nèi)皮細(xì)胞
是否是腫瘤細(xì)胞： 0
物種來源： 牛
生長狀態(tài)： 貼壁生長
組織來源： vascular endothelium
器官來源： 心臟
細(xì)胞形態(tài)： 內(nèi)皮樣
數(shù)量： 大量
年限： fetus
ATCC Number： CRL-1395?
運(yùn)輸方式： 凍存運(yùn)輸
FBHE細(xì)胞相關(guān)**： 正常
Designations: FBHE
Depositors: DJ Gospodarowicz
Biosafety Level: 2 [Cells contain Bovine Viral Diarrhea Virus (BVDV) ]
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Bos taurus
Morphology: endothelial
Source: Organ: heart
Tissue: vascular endothelium
Disease: normal
Cell Type: endothelial
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Age: FBHE細(xì)胞fetus
Comments: The FBHE cell line absolutely requires fibroblast growth factor (FGF) for survival, which can be supplied in the form of purified FGF or Bovine Pituitary Extract (BPE).
The line is positive for bovine viral diarrhea virus (BVD).
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium:
50 ?g/ml Bovine Pituitary Extract (as a source of fibroblast growth factor )
10%Colorado Calf Serum (Colorado Serum Company cat# CS1334)
Temperature: 37.0°C
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:5 is recommended
Medium Renewal: Twice per week
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. FBHE細(xì)胞Allow the flask to sit at room temperature (or at 37C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Subculture every 5 to 7 days , before 90% confluency.
Preservation: culture medium 95%; DMSO, 5%
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
References: 22883: Bolin SR, et al. Survey of cell lines in the American Type Culture Collection for bovine viral diarrhea virus. J. Virol. Methods 48: 211-221, 1994. PubMed: 7989438
23258: Gospodarowicz D, et al. Clonal growth of bovine vascular endothelial cells: fibroblast growth factor as a survival agent. Proc. Natl. Acad. Sci. USA 73: 4120-4124, 1976.FBHE細(xì)胞 PubMed: 1069301
