DATK32細胞
ATCC Number： HB-294?
是否是腫瘤細胞： 0
物種來源： 大鼠
細胞形態： **樣
**類型： IgG2a
數量： 大量
運輸方式： 凍存運輸
生長狀態： 懸浮生長
Designations: DATK32
Depositors: EC Butcher, DP Andrew
Isotype: IgG2a
DATK32細胞Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Rattus norvegicus (B cell); Mus musculus (myeloma) deposited as rat (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products: immunoglobulin; monoclonal antibody; against murine alpha 4, beta 7 integrin
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Comments: Animals were immunized with cells of the TK1 murine T cell lymphoma.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
The antibody reacts with the mouse lymphocyte mucosal homing receptor.
It is specific for mouse alpha 4, beta 7 heterodimers, and can induce alpha 4, beta 7 dependent lymphocyte aggregation.
The antibody inhibits alpha 4, beta 7 mediated adhesion activities including binding to MAdCAM-1, and alpha 4, beta 7 dependent VCAM-1 and fibronectin binding.
DATK32 is useful in flow cytometry methods using sensitive second stage reagents, but is ineffective in immunohistological staining.
Propagation: ATCC complete growth medium: RPMI 1640 medium with 4.5 g/L glucose,15 mM HEPES, 2 mM L-glutamine, 0.01 mM nonessential amino acids and 0.05 mM 2-mercaptoethanol, 90%; fetal bovine serum, 10%
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. DATK32細胞Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Preservation: Culture medium, 95%; DMSO, 5%
References: 22636: Berlin C, et al. Alpha 4 beta 7 integrin mediates lymphocyte binding to the mucosal vascular addressin MAdCAM-1. Cell 74: 185-195, 1993. PubMed: 7687523
23486: DATK32細胞Andrew DP, et al. Distinct but overlapping epitopes are involved in alpha 4 beta 7- mediated adhesion to vascular cell adhesion molecule-1, mucosal addressin-1, fibronectin, and lymphocyte aggregation. J. Immunol. 153: 3847-3861, 1994. PubMed: 7523506
