Schneider's Drosophila Line 2 [D. Mel. (2), SL2]細(xì)胞

器官來源： 胚胎

年限： embryo; 20 to 24 hours

數(shù)量： 大量

生長狀態(tài)： 懸浮生長

運輸方式： 凍存運輸

ATCC Number： CRL-1963?

是否是腫瘤細(xì)胞： 0

物種來源： 果蠅

細(xì)胞形態(tài)： 上皮樣

Designations: Schneider's Drosophila Line 2 [D. Mel. (2), SL2]

Schneider's Drosophila Line 2 [D. Mel. (2), SL2]細(xì)胞Depositors: IR Schneider

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension or loosely adherent

Organism: Drosophila melanogaster

Morphology: epithelial

Source: Organ: embryo

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Isolation: Isolation date: 1969

Applications: transfection host (Roche Transfection Reagents)

Age: embryo; 20 to 24 hours

Comments: Schneider's Drosophila Line 2 [D. Mel. (2), SL2]細(xì)胞The cells have been shown to support the growth of the insect stages of malaria parasites [Pubmed ID: 1734521].

Propagation: ATCC complete growth medium: Schneider's Drosophila Medium, 90%; heat-inactivated fetal bovine serum, 10%. NOTE: the fetal bovine serum should be selected for ability to support the growth of insect cell lines.

This medium is formulated for use without CO2.

Temperature: 23.0°C; (Max. 24.0 °C, Min. 22.0°C)

Note: The cells will grow as a loose monolayer (or in suspension) at any temperature between 16.0°C and 28.0°C.

Subculturing: Protocol: Gently resuspend the cells by pipetting medium across the monolayer or (for larger flasks) by slapping the flask against the base of the palm. Schneider's Drosophila Line 2 [D. Mel. (2), SL2]細(xì)胞Dilute the cell suspension to the desired concentration with fresh medium.

Maintain cultures at a cell concentration between 5 X 10(4) and 4 X 10(5) cells/cm2.

Subcultivation Ratio: A subcultivation ratio of 1:10 or greater is recommended

Medium Renewal: At the time of subcultivation

Preservation: Freeze medium: Complete growth medium 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor phase

Doubling Time: approximately 67 hours

References: 22960: Warburg A, Miller LH. Schneider's Drosophila Line 2 [D. Mel. (2), SL2]細(xì)胞Sporogonic development of a malaria parasite in vitro. Science 255: 448-450, 1992. PubMed: 1734521

22983: Schneider I. Cell lines derived from late embryonic stages of Drosophila melanogaster. J. Embryol. Exp. Morphol. 27: 353-365, 1972. PubMed: 4625067