AHH-1細胞

生長狀態： 懸浮生長

數量： 大量

細胞形態： **樣

年限： 33 years

細胞類型： B**細胞

運輸方式： 凍存運輸

是否是腫瘤細胞： 0

物種來源： 人

器官來源： 外周血

ATCC Number： CRL-8146?

Designations: AHH-1

Depositors: Massachusetts Institute of Technology

Biosafety Level: 2 [Cells Contain HERPESVIRUS ]

Shipped: frozen

Medium & Serum: See Propagation

AHH-1細胞Growth Properties: suspension

Organism: Homo sapiens

Morphology: lymphoblast

Source: Organ: peripheral blood

Cell Type: B lymphocyte;

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DNA Profile (STR): Amelogenin: X,Y

CSF1PO: 10

D13S317: 11,13

D16S539: 10

D5S818: 12,13

AHH-1細胞D7S820: 10,11

THO1: 6,9.3

TPOX: 8,9

vWA: 18,19

Cytogenetic Analysis: near diploid

Age: 33 years

Gender: male

Ethnicity: AHH-1細胞Caucasian

Comments: The cells have a high level of oxidative activity, and and can be used in mutagenesis assays for substances that are metabolized into mutagenic products. They are are sensitive to killing by benzo[a]pyrene. The cells carry an endogenous Epstein-Barr virus (EBV) genome and are EBNA positive.

Propagation: ATCC complete growth medium: RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate, 90%; horse serum, 10%

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Growth Conditions: The cells grow as floating aggregates of large cell clusters.

Subculturing: Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 X 10(5) viable cells/ml. Maintain cell density between 1 X 10(5) and 1 X 10(6) viable cells/ml.

Medium Renewal: 2 to 3 times per week

Preservation: Freeze medium: culture medium 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor phase

Doubling Time: 16 to 19 hrs

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001

parental cell line:AHH-1細胞ATCC CCL-156

derivative:ATCC CRL-10575

References: 21904: Crespi CL, Thilly WG. Mutation assays involving blood cells that metabolize toxic substances. US Patent 4,532,204 dated Jul 30 1985