145-2C11細胞
運輸方式： 凍存運輸
ATCC Number： CRL-1975?
數量： 大量
細胞形態： **樣
**類型： IgG , IgG
生長狀態： 懸浮生長
是否是腫瘤細胞： 0
物種來源： 倉鼠
Designations: 145-2C11
Depositors: JA Bluestone
Isotype: IgG , IgG
Biosafety Level: 1
Shipped: frozen
145-2C11細胞Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Cricetulus migratorius (B cell); Mus musculus (myeloma) deposited as hamster, Armenian (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products: immunoglobulin; monoclonal antibody; against mouse CD3
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Applications: 145-2C11細胞It reacts with all mature T cells and can both activate and inhibit T cell function.
The antibody is specific for a 25000 dalton protein component (CD3 epsilon) of the antigen specific T cell receptor.
The antibody reacts with the murine T cell receptor (CD3 - T3) complex.
Comments: Animals were immunized with the BM10-37 mouse cytotoxic T lymphocyte (CTL) cell clone (anti H-2 Kb).
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
The antibody reacts with the murine T cell receptor (CD3 - T3) complex.
The antibody is specific for a 25000 dalton protein component (CD3 epsilon) of the antigen specific T cell receptor.
145-2C11細胞It reacts with all mature T cells and can both activate and inhibit T cell function.
The antibody does not react with peripheral blood lymphocytes from rats, rabbits, miniature swine or hamsters.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Iscove's Modified Dulbecco's Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Subculturing: Protocol: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Medium Renewal: Every 2 to 3 days
Preservation: Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2005
recommended serum:ATCC 30-2020
References: 23298: Leo O, et al. Identification of a monoclonal antibody specific for a murine T3 polypeptide. Proc. Natl. Acad. Sci. USA 84: 1374-1378, 1987. PubMed: 2950524
28992: Kayagaki N, et al. 145-2C11細胞Polymorphism of murine Fas ligand that affects the biological activity. Proc. Natl. Acad. Sci. USA 94: 3914-3919, 1997. PubMed: 9108079
